AF/LE Purified Anti-Mouse CD3 Antibody[17A2]
![AF/LE Purified Anti-Mouse CD3 Antibody[17A2]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性: FCM
- 亚型: Rat IgG2b, κ
- 宿主: Rat
Alternate Names | CD3, CD3E/D/G/Z, CD3e/d/g/z, T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain |
Clone No | |
Isotype | Rat IgG2b, κ |
Leadtime |
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Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
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Background |
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
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Immunogen | Recombinant Mouse CD3 protein |
Abbre | CD3 |
Swissprot | |
Host | Rat |
Reactivity | Mouse |
Clonality | Monoclonal |
Purification Method | >98%, Protein A/G purified |
Applications | |
Research Areas | Immunology |
Concentration | ≥ 1 mg/mL |
Conjugation | None (AF/LE) |
Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
Expiration Date | 12个月 |
Shipping |
Ice bag |
C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD3 Antibody[17A2] (Right) and 0.2 μg Rat IgG2b, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody, then anti-Mouse CD8 FITC-conjugated Monoclonal Antibody.
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