AF/LE Purified Anti-Mouse CD3e Antibody[500A2]
![AF/LE Purified Anti-Mouse CD3e Antibody[500A2]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性： FCM
- 亚型： Syrian Hamster IgG
- 宿主： Syrian Hamster
| Alternate Names | T3E, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, Cd3e, T3, CD3epsilon |
| Clone No | |
| Isotype | Syrian Hamster IgG |
| Leadtime |
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| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
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| Background |
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region
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| Immunogen | Recombinant Mouse CD3e protein |
| Abbre | CD3e |
| Swissprot | |
| Host | Syrian Hamster |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Applications | |
| Concentration | ≥ 1 mg/mL |
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12个月 |
| Shipping |
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C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD3e Antibody[500A2] (Right) and 0.2 μg Syrian Hamster IgG, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti- Syrian Hamster IgG Secondary Antibody, then anti-Mouse CD8a FITC-conjugated Monoclonal Antibody.
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