AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]
![AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性: FCM
- 亚型: Rat IgG2b, κ
- 宿主: Rat
Alternate Names | CD54, Icam-1, Icam1, Intercellular adhesion molecule 1, MALA-2, MyD10 |
Clone No | |
Isotype | Rat IgG2b, κ |
Leadtime |
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Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
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Background |
CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.
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Immunogen | Recombinant Mouse CD54 protein |
Abbre | CD54 |
Swissprot | |
Host | Rat |
Reactivity | Mouse |
Clonality | Monoclonal |
Purification Method | >98%, Protein A/G purified |
Applications | |
Research Areas | Cell Adhesion;Cell Biology;Costimulatory Molecules;Immunology;Innate Immunity;Neuroscience;Neuroscience Cell Markers;Stem Cells |
Concentration | ≥ 1 mg/mL |
Conjugation | None (AF/LE) |
Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
Expiration Date | 12个月 |
Shipping |
Ice bag |
C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4] (Right) and 0.2 μg Rat IgG2b, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody.