ALD(Aldosterone) ELISA Kit
中文名称：醛固酮(ALD)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用ALD抗原包被于酶标板上，实验时样品或标准品中的ALD与包被的ALD竞争生物素标记的抗ALD单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，ALD浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中ALD的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的ALD，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Circadian light/dark cycle reversal exacerbates the progression of chronic kidney disease in mice
IF: 8.300 -
Mechanism of Folium Polygoni Cuspidati in liver-yang-hyperactivity hypertension based on network pharmacology,molecular docking and experimental pharmacological validation
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Low-intensity focused ultrasound combined with microbubbles for non-invasive downregulation of rabbit carotid body activity in the treatment of hypertension
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Defective natriuresis contributes to hyperkalemia in db/db mice during potassium supplementation
IF: 3.300Journal： JOURNAL OF HYPERTENSION (2024)Reactivity：MouseSample Type：Plasma -
Study on material basis and anti-hypertensive metabolomics of Zhengan-Xifeng-Tang(ZXT): A comparison between ZXT decoction and granules
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N-Methyl-d-Aspartate-Induced Excitotoxicity and Its Impact on the Renin–Angiotensin System in Retinal Tissue
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Renal Denervation Ameliorates Cardiomyocyte Apoptosis in Myocardial Ischemia–Reperfusion Injury Through Regulating Mitochondria–Endoplasmic Reticulum Contact
IF: 1.400Journal： Anatolian Journal of Cardiology (2024)Reactivity：RatSample Type：Serum -
Effects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats
IF: 0.700Journal： Natural Products Journal (2024)Reactivity：RatSample Type：Serum -
Autophagic degradation of MVBs in LSECs promotes Aldosterone induced-HSCs activation
IF: 6.600 -
Activation of Kir4.1/Kir5.1 contributes to the cyclosporin A-induced stimulation of the renal NaCl cotransporter and hyperkalemic hypertension
IF: 6.300
实验操作视频
