ALD(Aldosterone) ELISA Kit
中文名称：醛固酮(ALD)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用ALD抗原包被于酶标板上，实验时样品或标准品中的ALD与包被的ALD竞争生物素标记的抗ALD单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，ALD浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中ALD的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的ALD，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Histone deacetylase inhibitor, mocetinostat, regulates cardiac remodelling and renin-angiotensin system activity in rats with transverse aortic constriction-induced pressure overload cardiac hypertrophy.
IF: 2.930 -
Anti-hyperglycaemic effects of dioxidovanadium complex cis-[VO2(obz)py] avert kidney dysfunction in streptozotocin-induced diabetic male Sprague–Dawley rats
IF: 2.273Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2021)PMID：33759555Reactivity：RatSample Type：plasma -
Ameliorative Effects of Bredemolic Acid on Markers Associated with Renal Dysfunction in a Diet-Induced Prediabetic Rat Model
IF: 5.076Journal： Oxidative Medicine and Cellular Longevity (2020)PMID：32655765Reactivity：RatSample Type：Plasma,Urine,Tissue homogenate -
Amelioration of risk factors associated with diabetic nephropathy in diet-induced pre-diabetic rats by an uracil-derived diimine ruthenium(II) compound
IF: 4.545Journal： BIOMEDICINE & PHARMACOTHERAPY (2020)PMID：32768965Reactivity：RatSample Type：Plasma,Tissue homogenate -
Allopurinol and valproic acid improve cardiac triglyceride and Na+-K+-ATPase activity independent of circulating aldosterone in female rats with glucose intolerance
IF: 2.575Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020)PMID：32447998Reactivity：RatSample Type：plasma -
Cardio-protective effects of a dioxidovanadium(V) complex in male sprague–dawley rats with streptozotocin-induced diabetes
IF: 2.479 -
Maternal high-fructose intake during pregnancy and lactation induces metabolic syndrome in adult offspring
IF: 1.792 -
Maternal High-Fructose Intake Induces Multigenerational Activation of the Renin-Angiotensin-Aldosterone System
IF: 7.017 -
The effect of eplerenone on the renin-angiotensin-aldosterone system of rats with thyroid dysfunction
IF: 2.390 -
Aliskiren, Fosinopril, and Their Outcome on Renin-Angiotensin-Aldosterone System (RAAS) in Rats with Thyroid Dysfunction
IF: 2.287Journal： International Journal of Endocrinology (2019)PMID：31396276Reactivity：RatSample Type：serum
实验操作视频
