ALD(Aldosterone) ELISA Kit
中文名称：醛固酮(ALD)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用ALD抗原包被于酶标板上，实验时样品或标准品中的ALD与包被的ALD竞争生物素标记的抗ALD单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，ALD浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中ALD的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的ALD，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats
IF: 2.276Journal： TOXICOLOGY MECHANISMS AND METHODS (2019)PMID：30426860Reactivity：RatSample Type：heart tissue -
Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system
IF: 2.107 -
Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats
Journal： Chinese Herbal Medicines (2019)PMID：36121411Reactivity：RatSample Type：Plasma,Serum,Tissue homogenate -
The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers
IF: 2.885 -
Neuroglobin correlates with cryptochrome-1 in obstructive sleep apnea with primary aldosteronism
IF: 2.766 -
Oral hormonal therapy with ethinylestradiol–levonorgestrel improves insulin resistance, obesity, and glycogen synthase kinase-3 independent of circulating mineralocorticoid in estrogen-deficient rats
IF: 2.210Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018)PMID：29400073Reactivity：RatSample Type：plasma -
Ameliorative effect of low-dose spironolactone on obesity and insulin resistance is through replenishment of estrogen in ovariectomized rats
IF: 2.210Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018)PMID：30422674Reactivity：RatSample Type：Plasma -
Blockade of mineralocorticoid receptor ameliorates oral contraceptive-induced insulin resistance by suppressing elevated uric acid and glycogen synthase kinase-3 instead of circulating mineralocorticoid
IF: 1.806Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2018)PMID：30318954Reactivity：RatSample Type：Plasma -
Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration
IF: 6.337 -
Microbiota Modulate Anxiety-Like Behavior and Endocrine Abnormalities in Hypothalamic-Pituitary-Adrenal Axis
IF: 4.300Journal： Frontiers in Cellular and Infection Microbiology (2017)PMID：29250490Reactivity：MouseSample Type：Tissue homogenate
实验操作视频
