BCA Protein Colorimetric Assay Kit
中文名称:BCA蛋白浓度测定试剂盒

Price:
Size:
500 Assays
96 T (80 samples)
- 样本类型: 血清(浆);培养液;动植物组织;细胞
- 检测仪器及波长: 酶标仪(540-590 nm,最佳检测波长为562 nm)
- 研究领域: 氨基酸与蛋白
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
蛋白质在碱性条件下将二价铜离子还原为一价铜离子,一价铜离子与 BCA 分子形成紫色络合物。此络合物可用540-590 nm波长检测,反应物的颜色和蛋白浓度在一定范围内具有线性关系。
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指标简称 | TP |
灵敏度 | 0.0165 mg/mL |
检测范围 | 0.0165-1 mg/mL |
精密度 | 批间差:4.5% | 批内差:2.2% |
样本量 | 20 μL |
检测时间 | 40 min |
检测仪器及波长 | 酶标仪(540-590 nm,最佳检测波长为562 nm) |
保存条件 | 该产品可在 室温避光条件下保存12个月。 |
有效期 | 12个月 |
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Q1:小鼠血清检测蛋白浓度大概测值多少?推荐稀释多少倍?
小鼠血清蛋白浓度参考值为50-70g/L,参考稀释倍数60-80倍。由于生物样本间差异性,以上数据仅供参考。