cAMP(Cyclic adenosine monophosphate) ELISA Kit
中文名称：环磷酸腺苷(cAMP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的cAMP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Osthole prevents tamoxifen-induced liver injury in mice
IF: 3.562Journal： ACTA PHARMACOLOGICA SINICA (2018)PMID：30315252Reactivity：MouseSample Type：Tissue homogenate -
CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells
IF: 3.333Journal： INTERNATIONAL JOURNAL OF ONCOLOGY (2018)PMID：30535454Reactivity：HumanSample Type：Cell lysate -
A novel role and mechanism of cystic fibrosis transmembrane conductance regulator in bisphenol A-induced prostate cancer
IF: 2.959Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2018)PMID：30485539Reactivity：HumanSample Type：Cell culture supernatant -
rLj-RGD3, a novel recombinant toxin protein from Lampetra japonica, prevents coronary thrombosis-induced acute myocardial infarction by inhibiting platelet functions in rats
IF: 2.559Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018)PMID：29407168Reactivity：RatSample Type：platelets -
Identification of a novel GNAS mutation in a case of pseudohypoparathyroidism type 1A with normocalcemia
IF: 1.913Journal： BMC Medical Genetics (2018)PMID：30060753Reactivity：OpossumSample Type：Cell culture supernatant -
Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin?angiotensin system activity
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30402152Reactivity：RatSample Type：Plasma,Tissue homogenate -
Biatriosporin D displays anti-virulence activity through decreasing the intracellular cAMP levels
IF: 3.791 -
Osthole pretreatment alleviates TNBS-induced colitis in mice via both cAMP/PKA-dependent and independent pathways
IF: 3.223 -
Analysis and application of a neutralizing linear epitope on liable toxin B of enterotoxin Escherichia coli
IF: 3.337
实验操作视频
