cAMP(Cyclic adenosine monophosphate) ELISA Kit
中文名称：环磷酸腺苷(cAMP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的cAMP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Royal Jelly Alleviates Cognitive Deficits and β-Amyloid Accumulation in APP/PS1 Mouse Model Via Activation of the cAMP/PKA/CREB/BDNF Pathway and Inhibition of Neuronal Apoptosis
IF: 3.633Journal： Frontiers in Aging Neuroscience (2019)PMID：30687079Reactivity：MouseSample Type：Tissue homogenate -
Calcimimetic R568 improved cardiac remodeling by classic and novel renin-angiotensin system in spontaneously hypertensive rats
IF: 3.005 -
Effect of low androgen status on the expression of adenosine A2A and A2B receptors in rat penile corpus cavernosum
IF: 1.840 -
The Inhibition by Guanfu Base A of Neuropathic Pain Mediated by P2Y12 Receptor in Dorsal Root Ganglia
IF: 4.211Journal： ACS Chemical Neuroscience (2018)PMID：30475578Reactivity：RatSample Type：Cell culture supernatant -
Developmental Exposure to Atrazine Impairs Spatial Memory and Downregulates the Hippocampal D1 Dopamine Receptor and cAMP-Dependent Signaling Pathway in Rats
IF: 3.687Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2018)PMID：30065202Reactivity：RatSample Type：Tissue homogenate -
Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice
IF: 3.563Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2018)PMID：29499209Reactivity：MouseSample Type：liver -
Osthole prevents tamoxifen-induced liver injury in mice
IF: 3.562Journal： ACTA PHARMACOLOGICA SINICA (2018)PMID：30315252Reactivity：MouseSample Type：Tissue homogenate -
CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells
IF: 3.333Journal： INTERNATIONAL JOURNAL OF ONCOLOGY (2018)PMID：30535454Reactivity：HumanSample Type：Cell lysate -
A novel role and mechanism of cystic fibrosis transmembrane conductance regulator in bisphenol A-induced prostate cancer
IF: 2.959Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2018)PMID：30485539Reactivity：HumanSample Type：Cell culture supernatant -
rLj-RGD3, a novel recombinant toxin protein from Lampetra japonica, prevents coronary thrombosis-induced acute myocardial infarction by inhibiting platelet functions in rats
IF: 2.559Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018)PMID：29407168Reactivity：RatSample Type：platelets
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