cAMP(Cyclic adenosine monophosphate) ELISA Kit
中文名称：环磷酸腺苷(cAMP)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的cAMP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：MouseSample Type：prefrontal cortex (PFC) tissueGlucose and HODEs regulate Aspergillus ochraceus quorum sensing through the GprC-AcyA pathway
IF: 6.200Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2024)Reactivity：Aspergillus ochraceusHigh Stretch Modulates cAMP/ATP Level in Association with Purine Metabolism via miRNA–mRNA Interactions in Cultured Human Airway Smooth Muscle Cells
IF: 6.000Deletion of GPR81 activates CREB/Smad7 pathway and alleviates liver fibrosis in mice
IF: 6.000Journal： MOLECULAR MEDICINE (2024)Reactivity：MouseSample Type：liver tissueAn-Gong-Niu-Huang-Wan (AGNHW) regulates cerebral blood flow by improving hypoperfusion, cerebrovascular reactivity and microcirculation disturbances after stroke
IF: 5.300Journal： Chinese Medicine (2024)Reactivity：MouseSample Type：serum,brain homogenatesNatriuretic peptide receptor-C perturbs mitochondrial respiration in white adipose tissue
IF: 5.0002′-O-Galloylhyperin Prevents Tissue Remodeling in Thyroid Eye Disease: Prospects as a Thyrotropin Receptor Antagonist
IF: 5.000Journal： JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM (2024)Reactivity：HumanThe Activation of p300 Enhances the Sensitivity of Pituitary Adenomas to Dopamine Agonist Treatment by Regulating the Transcription of DRD2
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：Rat,MouseRoflupram alleviates autophagy defects and reduces mutant hSOD1-induced motor neuron damage in cell and mouse models of amyotrophic lateral sclerosis
IF: 4.700Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviate Brain Damage Following Subarachnoid Hemorrhage via the Interaction of miR-140-5p and HDAC7
IF: 4.600Journal： MOLECULAR NEUROBIOLOGY (2024)Reactivity：MouseSample Type：brain tissue
