cAMP(Cyclic adenosine monophosphate) ELISA Kit

中文名称：环磷酸腺苷(cAMP)酶联免疫吸附测定试剂盒

货号：E-EL-0056
文献（123）
说明书

说明书

别称：

3'-5'-cyclic adenosine monophosphate、CAMP、Cyclic AMP、Cyclic adenosine monophosphate

cAMP(Cyclic adenosine monophosphate) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Universal
检测范围： 1.56-100 ng/mL
灵敏度： 0.94 ng/mL

产品应用	ELISA
检测原理	本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。
反应类型	Competitive-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	2 h 30 min
反应性	Universal
检测方法	比色法;ELISA;竞争法
检测范围	1.56-100 ng/mL
灵敏度	0.94 ng/mL
样本体积	50 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的cAMP，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Mitigation of Myocardial Ischemia/Reperfusion–induced Chronic Heart Failure via Shexiang Baoxin Pill–Mediated Regulation of the S1PR1 Signaling Pathway

IF: 7.9

Journal： PHYTOMEDICINE (2024)

DOI：10.1016/j.phymed.2024.155390

PMID：38569296

Reactivity：Rat,Human
Exploring the mechanism of Paotianxiong polysaccharide in the treatment of chronic kidney disease combining metabolomics and microbiomics technologies

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.138629

Reactivity：Rat

Sample Type：serum
Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens

IF: 7.5

Journal： Cell Reports (2024)

DOI：10.1016/j.celrep.2024.114830

Reactivity：Human
Dexmedetomidine facilitates autophagic flux to promote liver regeneration by suppressing GSK3β activity in mouse partial hepatectomy

IF: 6.9

Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)

DOI：10.1016/j.biopha.2024.117038

Reactivity：Mouse

Sample Type：liver tissue
1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway

IF: 6.2

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)

DOI：10.1016/j.ecoenv.2024.116464

Reactivity：Mouse

Sample Type：prefrontal cortex (PFC) tissue
Glucose and HODEs regulate Aspergillus ochraceus quorum sensing through the GprC-AcyA pathway

IF: 6.2

Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2024)

DOI：10.1007/s00018-024-05160-z

Reactivity：Aspergillus ochraceus
High Stretch Modulates cAMP/ATP Level in Association with Purine Metabolism via miRNA–mRNA Interactions in Cultured Human Airway Smooth Muscle Cells

IF: 6.0

Journal： Cells (2024)

DOI：10.3390/cells13020110

PMID：38247802

Reactivity：Human
Deletion of GPR81 activates CREB/Smad7 pathway and alleviates liver fibrosis in mice

IF: 6.0

Journal： MOLECULAR MEDICINE (2024)

DOI：10.1186/s10020-024-00867-y

Reactivity：Mouse

Sample Type：liver tissue
An-Gong-Niu-Huang-Wan (AGNHW) regulates cerebral blood flow by improving hypoperfusion, cerebrovascular reactivity and microcirculation disturbances after stroke

IF: 5.3

Journal： Chinese Medicine (2024)

DOI：10.1186/s13020-024-00945-7

Reactivity：Mouse

Sample Type：serum,brain homogenates
Natriuretic peptide receptor-C perturbs mitochondrial respiration in white adipose tissue

IF: 5.0

Journal： JOURNAL OF LIPID RESEARCH (2024)

DOI：10.1016/j.jlr.2024.100623

Reactivity：Mouse

Species

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