cAMP(Cyclic adenosine monophosphate) ELISA Kit
中文名称：环磷酸腺苷(cAMP)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的cAMP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Cardiac energy depletion in a rat model of polycystic ovarian syndrome is reversed by acetate and associated with inhibitory effect of HDAC2/mTOR
IF: 5.000Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2023)PMID：38048978Reactivity：RatSample Type：cardiac tissueAspirin Suppresses Hepatic Glucagon Signaling Through Decreasing Production of Thromboxane A2
IF: 4.800Serotonin/5-HT7 receptor provides an adaptive signal to enhance pigmentation response to environmental stressors through cAMP-PKA-MAPK, Rab27a/RhoA, and PI3K/AKT signaling pathways
IF: 4.800Mechanism of Xiaoyao San in treating non-alcoholic fatty liver disease with liver depression and spleen deficiency: based on bioinformatics, metabolomics and in?vivo experiments
IF: 4.400Journal： JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2023)PMID：37440274Reactivity：RatSample Type：serumcAMP Agonist Forskolin Disrupts Mitochondrial Metabolism and Induces Senescence in Human Mesenchymal Cells
IF: 4.000Heme and Cu2+-induced vasoactive intestinal peptide (VIP) tyrosine nitration: A possible molecular mechanism for the attenuated anti-inflammatory effect of VIP in inflammatory diseases
IF: 3.900Potential mechanism of Qinggong Shoutao pill alleviating age-associated memory decline based on integration strategy
IF: 3.800Assessing the Adverse Impacts of PM2.5 on Olfactory System Using an Air-liquid Interface Culture Model of Primary Olfactory Epithelial Cells
IF: 3.100Journal： CHEMICAL RESEARCH IN CHINESE UNIVERSITIES (2023)Reactivity：MouseAcetate mitigates cardiac mitochondrial dysfunction in experimental model of polycystic ovarian syndrome by modulating GPCR41/43 and PROKR1
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：37757668Reactivity：RatSample Type：cardiacThe gut microbe Bacteroides fragilis ameliorates renal fibrosis in mice
IF: 17.694
