cAMP(Cyclic adenosine monophosphate) ELISA Kit

中文名称：环磷酸腺苷(cAMP)酶联免疫吸附测定试剂盒

货号：E-EL-0056
文献（115）
说明书

说明书

别称：

3'-5'-cyclic adenosine monophosphate、CAMP、Cyclic AMP、Cyclic adenosine monophosphate

cAMP(Cyclic adenosine monophosphate) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Universal
检测范围： 1.56-100 ng/mL
灵敏度： 0.94 ng/mL

产品应用	ELISA
检测原理	本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。
反应类型	Competitive-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	2 h 30 min
反应性	Universal
检测方法	比色法;ELISA;竞争法
检测范围	1.56-100 ng/mL
灵敏度	0.94 ng/mL
样本体积	50 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的cAMP，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

cAMP Agonist Forskolin Disrupts Mitochondrial Metabolism and Induces Senescence in Human Mesenchymal Cells

IF: 4.000

Journal： STEM CELLS AND DEVELOPMENT (2023)

DOI：10.1089/scd.2022.0180

PMID：36503249

Reactivity：Human
Heme and Cu2+-induced vasoactive intestinal peptide (VIP) tyrosine nitration: A possible molecular mechanism for the attenuated anti-inflammatory effect of VIP in inflammatory diseases

IF: 3.9

Journal： BIOCHIMIE (2023)

DOI：10.1016/j.biochi.2023.07.011

PMID：37481062

Reactivity：Human
Potential mechanism of Qinggong Shoutao pill alleviating age-associated memory decline based on integration strategy

IF: 3.8

Journal： PHARMACEUTICAL BIOLOGY (2023)

DOI：10.1080/13880209.2023.2291689

PMID：38145345

Reactivity：Mouse

Sample Type：hippocampal tissue
Assessing the Adverse Impacts of PM2.5 on Olfactory System Using an Air-liquid Interface Culture Model of Primary Olfactory Epithelial Cells

IF: 3.100

Journal： CHEMICAL RESEARCH IN CHINESE UNIVERSITIES (2023)

DOI：10.1007/s40242-023-3019-z

Reactivity：Mouse
Acetate mitigates cardiac mitochondrial dysfunction in experimental model of polycystic ovarian syndrome by modulating GPCR41/43 and PROKR1

IF: 3.1

Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)

DOI：10.1016/j.bbrc.2023.09.061

PMID：37757668

Reactivity：Rat

Sample Type：cardiac
The gut microbe Bacteroides fragilis ameliorates renal fibrosis in mice

IF: 17.694

Journal： Nature Communications (2022)

DOI：10.1038/s41467-022-33824-6

PMID：36241632

Reactivity：Mouse

Sample Type：renal
Combining adoptive NK cell infusion with a dopamine-releasing peptide reduces senescent cells in aged mice

IF: 9.685

Journal： Cell Death & Disease (2022)

DOI：10.1038/s41419-022-04562-w

PMID：35383143

Reactivity：Mouse
Muscone and (+)-Borneol Cooperatively Strengthen CREB Induction of Claudin 5 in IL-1β-Induced Endothelium Injury

IF: 7.675

Journal： Antioxidants (2022)

DOI：10.3390/antiox11081455

PMID：35892657

Reactivity：Mouse
Forskolin, an Adenylcyclase/cAMP/CREB Signaling Activator Restoring Myelin-Associated Oligodendrocyte Destruction in Experimental Ethidium Bromide Model of Multiple Sclerosis

IF: 7.666

Journal： Cells (2022)

DOI：10.3390/cells11182771

PMID：36139346

Reactivity：Rat

Sample Type：brain tissue
Phosphodiesterase 4B activation exacerbates pulmonary hypertension induced by intermittent hypoxia by regulating mitochondrial injury and cAMP/PKA/p-CREB/PGC-1α signaling

IF: 7.419

Journal： BIOMEDICINE & PHARMACOTHERAPY (2022)

DOI：10.1016/j.biopha.2022.114095

PMID：36495666

Reactivity：Rat

Sample Type：serum

Species

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Universal

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