cGMP(Cyclic GMP) ELISA Kit
中文名称：环磷酸鸟苷(cGMP)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Universal
- 检测范围： 0.78-50 pmol/mL
- 灵敏度： 0.47 pmol/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用cGMP抗原包被于酶标板上，实验时样品或标准品中的cGMP与包被的cGMP竞争生物素标记的抗cGMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cGMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cGMP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.78-50 pmol/mL |
| 灵敏度 | 0.47 pmol/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的cGMP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Stem cell–homing biomimetic hydrogel promotes the repair of osteoporotic bone defects through osteogenic and angiogenic coupling
IF: 11.700Novel Angiogenesis Role of GLP-1(32–36) to Rescue Diabetic Ischemic Lower Limbs via GLP-1R–Dependent Glycolysis in Mice
IF: 8.700Exploring the mechanism of Paotianxiong polysaccharide in the treatment of chronic kidney disease combining metabolomics and microbiomics technologies
IF: 7.700Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity：RatSample Type：serumExploring PDE5A upregulation in bipolar disorder: insights from single-nucleus RNA sequencing of human basal ganglia
IF: 5.800Journal： Translational Psychiatry (2024)Reactivity：MouseSample Type：striatal tissueNatriuretic peptide receptor-C perturbs mitochondrial respiration in white adipose tissue
IF: 5.000An angiotensin-converting enzyme inhibitory peptide LVLPGE exerts an antihypertensive effect and improves endothelial function via eNOS/NO/cGMP pathway
IF: 4.800Upregulation of phosphodiesterase 7A leads to the downregulation of cAMP-PKA-CREB-BDNF signaling and neuroinflammation in the hippocampus and contributes to concurrent chronic pain and depression in two mouse models
IF: 4.500Journal： INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY (2024)Reactivity：MouseSample Type：hippocampus tissueNitric oxide facilitates the S‐nitrosylation and deubiquitination of Notch1 protein to maintain cancer stem cells in human NSCLC
IF: 4.300Chronic sleep deprivation induces erectile dysfunction through increased oxidative stress, apoptosis, endothelial dysfunction, and corporal fibrosis in a rat model
IF: 3.300Journal： Journal of Sexual Medicine (2024)Reactivity：RatSample Type：penile tissueAqueous extract of Peristrophe bivalvis (L.) Merr. leaf reversed the detrimental effects of nitric oxide synthase inhibitor on blood lipid profile and glucose level
IF: 2.900
