cGMP(Cyclic GMP) ELISA Kit
中文名称：环磷酸鸟苷(cGMP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.78-50 pmol/mL
- 灵敏度： 0.47 pmol/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用cGMP抗原包被于酶标板上，实验时样品或标准品中的cGMP与包被的cGMP竞争生物素标记的抗cGMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cGMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cGMP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.78-50 pmol/mL |
| 灵敏度 | 0.47 pmol/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的cGMP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Inhibitory effect of (pro)renin receptor decoy inhibitor PRO20 on endoplasmic reticulum stress during cardiac remodeling
IF: 5.988 -
Ajuga bracteosa Exerts Antihypertensive Activity in l-NAME-Induced Hypertension Possibly through Modulation of Oxidative Stress, Proinflammatory Cytokines, and the Nitric Oxide/Cyclic Guanosine Monophosphate Pathway
IF: 4.132 -
Dexlansoprazole prevents pulmonary artery hypertension by inhibiting pulmonary artery smooth muscle cell to fibroblast transition
IF: 3.940Journal： American Journal of Translational Research (2022)DOI：PMID:36105026PMID：36105026Reactivity：RatSample Type：plasma -
L-Arginine reverses maternal and pre-pubertal codeine exposure-induced sexual dysfunction via upregulation of androgen receptor gene and NO/cGMP signaling
IF: 3.752 -
Prenatal chronic stress impairs the learning and memory ability via inhibition of the NO/cGMP/PKG pathway in the Hippocampus of offspring
IF: 3.352Journal： BEHAVIOURAL BRAIN RESEARCH (2022)PMID：35850398Reactivity：RatSample Type：hippocampal tissue -
FXYD3 promotes the proliferation, migration, and invasion of pancreatic cancer cells by regulating the cGMP-PKG signaling pathway
IF: 1.718Journal： Molecular & Cellular Toxicology (2022)Reactivity：HumanSample Type：pancreatic tissue -
Inhibition of Phosphodiesterase 5 Promotes the Aromatase-Mediated Estrogen Biosynthesis in Osteoblastic Cells by Activation of cGMP/PKG/SHP2 Pathway
IF: 5.555 -
Protective effect of the combination of essential oil from patchouli and tangerine peel against gastric ulcer in rats
IF: 4.360 -
Ziziphus Oxyphylla hydro-methanolic extract ameliorates hypertension in L-NAME induced hypertensive rats through NO/cGMP pathway and suppression of oxidative stress related inflammatory biomarkers
IF: 4.360 -
Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet
IF: 4.310Journal： AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2021)PMID：34229480Reactivity：RatSample Type：serum, testis
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