cGMP(Cyclic GMP) ELISA Kit
中文名称:环磷酸鸟苷(cGMP)酶联免疫吸附测定试剂盒

Price:
- 反应性: Universal
- 检测范围: 0.78-50 pmol/mL
- 灵敏度: 0.47 pmol/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用cGMP抗原包被于酶标板上,实验时样品或标准品中的cGMP与包被的cGMP竞争生物素标记的抗cGMP单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,cGMP浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中cGMP的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Universal |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 0.78-50 pmol/mL |
灵敏度 | 0.47 pmol/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的cGMP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Sildenafil ameliorates leptin resistance and normalizes lipid handling in the hypothalamic and adipose tissues of testosterone-exposed pregnant rats
-
Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway
IF: 7.076 -
Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats
IF: 3.206 -
Aggravated endothelial endocrine dysfunction and intimal thickening of renal artery in high-fat diet-induced obese pigs following renal denervation
IF: 2.078Journal: BMC Cardiovascular Disorders (2020)PMID:32295540Reactivity:PigSample Type:Tissue homogenate -
Simultaneous inhibition of neprilysin and activation of ACE2 prevented diabetic cardiomyopathy
IF: 2.761Journal: Pharmacological Reports (2019)PMID:31470292Reactivity:RatSample Type:heart tissue,plasma -
Effect of low androgen status on the expression of adenosine A2A and A2B receptors in rat penile corpus cavernosum
IF: 1.840 -
Osthole prevents tamoxifen-induced liver injury in mice
IF: 3.562Journal: ACTA PHARMACOLOGICA SINICA (2018)PMID:30315252Reactivity:MouseSample Type:Tissue homogenate -
C-Type Natriuretic Peptide Ameliorates Lipopolysaccharide-Induced Cardiac Dysfunction in Rats with Pulmonary Arterial Hypertension
IF: 2.583 -
Osthole prevents acetaminophen-induced liver injury in mice
IF: 3.223Journal: ACTA PHARMACOLOGICA SINICA (2017)PMID:29022574Reactivity:MouseSample Type:Tissue homogenate

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