Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)
中文名称:肌酐(Cr)比色法测试盒(肌氨酸氧化酶法)

Price:
- 样本类型: 血清(浆);尿液
- 检测仪器及波长: 酶标仪(510-520 nm,最佳检测波长为515 nm)
- 研究领域: 肝肾功能
检测原理 |
肌酐在肌酐酶的催化下生成肌酸,肌酸在肌酸酶的催化下生成肌氨酸和尿素,肌氨酸再经肌氨酸氧化酶催化生成甘氨酸、甲醛和过氧化氢。过氧化氢与显色剂在过氧化物酶的催化下反应生成粉红色化合物,可通过测定515 nm波长下的OD值变化,来测定计算出肌酐的含量。
|
指标简称 | Cr |
灵敏度 | 3.8 μmol/L |
检测范围 | 20.45-400 μmol/L |
精密度 | 批间差:3.7% | 批内差:1.4% |
样本量 | 12 μL |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(510-520 nm,最佳检测波长为515 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存3个月。 |
有效期 | 3个月 |
-
Metabolomic and Cellular Mechanisms of Drug-Induced Ototoxicity and Nephrotoxicity: Therapeutic Implications of Uric Acid Modulation
IF: 14.3 -
Biomimetic carrier-free nanoplatform based on natural polyphenols and siRNA for targeted therapy in rheumatoid arthritis
IF: 13.3Journal: CHEMICAL ENGINEERING JOURNAL (2025)Reactivity:MouseSample Type:blood -
Carboxymethyl Poria cocos polysaccharides protect against septic kidney injury by regulating the Nrf2-NF-κB signaling pathway
IF: 7.7Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)Reactivity:MouseSample Type:Serum -
Associations of human exposure to 6PPD and 6PPDQ with colorectal cancer: A mixture analysis
IF: 7.6Journal: ENVIRONMENTAL POLLUTION (2025)Reactivity:HumanSample Type:Urinary -
Design and Synthesis of Hederagenin Derivatives for the Treatment of Sepsis by Targeting TAK1 and Regulating the TAK1-NF-κB/MAPK Signaling
IF: 6.8Journal: JOURNAL OF MEDICINAL CHEMISTRY (2025)Reactivity:MouseSample Type:serum,liver tissue,myocardial tissue -
TMEM45A enhances palbociclib resistance and cellular glycolysis by activating AKT/mTOR signaling pathway in HR+ breast cancer
IF: 6.1Journal: Cell Death Discovery (2025)Reactivity:MouseSample Type:Blood -
Hydrogel microneedles with multifunctional strategy for prolonged hyperuricemia management
IF: 6.1Journal: Journal of Materials Chemistry B (2025)Reactivity:MouseSample Type:serum -
Comparative Analysis of Hyperuricemia Induction Methods and Probiotic Interventions in Mice
IF: 5.8Journal: Current Research in Microbial Sciences (2025)Reactivity:MouseSample Type:Serum -
A novel oncolytic Vaccinia virus armed with IL-12 augments antitumor immune responses leading to durable regression in murine models of lung cancer
IF: 5.7Journal: Frontiers in Immunology (2025)Reactivity:MouseSample Type:Serum -
Iontophoresis-driven transdermal drug delivery system based on porous microneedles for hyperuricemia treatment
IF: 5.3Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS (2025)Reactivity:MouseSample Type:serum
E-BC-K183-M | Urea (BUN) Colorimetric Assay Kit (Urease Method) | 96 T (40 samples) |
E-BC-K016-M | Uric Acid (UA) Colorimetric Assay Kit | 96 T (80 samples) , 48 T (32 samples) |
Q1:请问该试剂盒中加入试剂二2min后测定第一次OD值A1的目的是什么呢,为什么需要求两测得差值呢?
由于样本本身含有肌酐和肌酸,加入试剂一之后,将样本中的肌酸转换成了过氧化氢,但是样本中的肌酐并没有在第一步反应,而是从第二步开始的;前面2 min的反应是为了消耗掉样本自身含有的肌酸,扣除这部分的测值,保证测定结果的准确性。
Q2:如果样本溶血,能设置对照排除干扰吗?
这个试剂盒对于溶血严重的样本设置对照效果不大,建议您还是重新取样,用没有溶血的样本进行实验。

暂无文件,请在搜索框输入识别码查询相关文件

名称 | 状态 | 有效期至 | 操作 | |
---|---|---|---|---|
{{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注:请在有效期内下载相关文件,过期后页面内将不提供文件下载及预览!