DA(Dopamine) ELISA Kit
中文名称：多巴胺(DA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用DA抗原包被于酶标板上，实验时样品或标准品中的DA与包被的DA竞争生物素标记的抗DA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，DA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中DA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的DA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Ribose-cysteine and levodopa abrogate Parkinsonism via the regulation of neurochemical and redox activities in alpha-synuclein transgenic Drosophila melanogaster models
IF: 1.200 -
Effects of a crude extract of Echinops mosulensis on an induced Parkinson’s disease model in mice
IF: 1.100Journal： PHARMACIA (2024) -
Functional associations of the gut microbiome with dopamine, serotonin, and BDNF in schizophrenia: a pilot study
IF: 1.100Journal： Egyptian Journal of Neurology Psychiatry and Neurosurgery (2024)Reactivity：HumanSample Type：plasma -
Combination anti-PD-1 and electroacupuncture induces a potent anti-tumor immune response in microsatellite-stable colorectal cancer
IF: 10.100 -
Inhibition of abnormal C/EBPβ/α-Syn signaling pathway through activation of Nrf2 ameliorates Parkinson's disease-like pathology
IF: 7.800Journal： AGING CELL (2023)PMID：37614147Reactivity：MouseSample Type：striatum,substantia nigra pars compacta (SNc) -
Apigetrin-enriched Pulmeria alba extract prevents assault of STZ on pancreatic β-cells and neuronal oxidative stress with concomitant attenuation of tissue damage and suppression of inflammation in the brain of diabetic rats
IF: 7.500 -
Echinacoside exerts antidepressant-like effects through enhancing BDNF-CREB pathway and inhibiting neuroinflammation via regulating microglia M1/M2 polarization and JAK1/STAT3 pathway
IF: 5.600 -
Ansofaxine hydrochloride inhibits tumor growth and enhances Anti-TNFR2 in murine colon cancer model
IF: 5.600 -
Withania somnifera ameliorates sexual arousal and impotence in stressed sexually sluggish male rats by modulating neurotransmitters and NO/cGMP/PDE5α pathway
IF: 5.400 -
Cotransplantation of NSCs and ethyl stearate promotes synaptic plasticity in PD rats by Drd1/ERK/AP-1 signaling pathway
IF: 5.400
实验操作视频
