DA(Dopamine) ELISA Kit
中文名称：多巴胺(DA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用DA抗原包被于酶标板上，实验时样品或标准品中的DA与包被的DA竞争生物素标记的抗DA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，DA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中DA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的DA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Hydrogen Alleviates Neuronal Injury and Neuroinflammation Induced by Microglial Activation via the Nuclear Factor Erythroid 2-related Factor 2 Pathway in Sepsis-associated Encephalopathy
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A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients
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LianXia Formula Granule Attenuates Cardiac Sympathetic Remodeling in Rats with Myocardial Infarction via the NGF/TrKA/PI3K/AKT Signaling Pathway
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34221073Reactivity：RatSample Type：plasma -
A novel resonance Rayleigh scattering aptasensor for dopamine detection based on an Exonuclease III assisted signal amplification by G - quadruplex nanowires formation
IF: 4.762Journal： Arabian Journal of Chemistry (2020)Reactivity：MouseSample Type：brain -
In Vivo Metabolic Response upon Exposure to Gold Nanorod Core/Silver Shell Nanostructures: Modulation of Inflammation and Upregulation of Dopamine
IF: 4.556Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)PMID：31936206Reactivity：MouseSample Type：Spleen -
Evaluation of Priming Efficiency of Forskolin in Tissue-Specific Human Mesenchymal Stem Cells into Dopaminergic Neurons: An In Vitro Comparative Study
IF: 4.366
实验操作视频
