DHT(Dihydrotestosterone) ELISA Kit
中文名称：双氢睾酮(DHT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 39.06-2500 pg/mL
- 灵敏度： 23.44 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用DHT抗原包被于酶标板上，实验时样品或标准品中的DHT与包被的DHT竞争生物素标记的抗DHT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，DHT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中DHT的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 39.06-2500 pg/mL |
| 灵敏度 | 23.44 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的DHT，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice
IF: 8.400Journal： Cell Communication and Signaling (2024)PMID：38491517Reactivity：MouseSample Type：plasma,prostate tissue -
Comparison and investigation on characteristics of polycystic ovary syndrome rat models induced by letrozole, testosterone propionate, and high-fat diets
IF: 3.700Journal： REPRODUCTIVE BIOMEDICINE ONLINE (2024)Reactivity：RatSample Type：Serum -
Bisphenol A-induced polycystic ovary syndrome (PCOS) with hormonal and metabolic implications in rats
IF: 3.300Journal： REPRODUCTIVE TOXICOLOGY (2024)Reactivity：RatSample Type：serum -
Dehydroepiandrosterone with a high-fat diet treatment at inducing polycystic ovary syndrome in rat model
IF: 2.100 -
Sex Differences and Cytokine Profiles among Patients Hospitalized for COVID-19 and during Their Recovery: The Predominance of Adhesion Molecules in Females and Oxidative Stress in Males
IF: 7.800 -
New Target for Minoxidil in the Treatment of Androgenetic Alopecia
IF: 4.800 -
Metabolites of gut microbiota fermenting Poria cocos polysaccharide alleviates chronic nonbacterial prostatitis in rats
IF: 8.025Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)PMID：35398386Reactivity：RatSample Type：serum -
A Combination of Natural Products, BenPros (Green Tea Extract, Soybean Extract and Camellia Japonica Oil), Ameliorates Benign Prostatic Hyperplasia
IF: 2.838 -
Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2
IF: 4.432
实验操作视频
