DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit
中文名称：1,25二羟基维生素D3(DHVD3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用DHVD3抗原包被于酶标板上，实验时样品或标准品中的DHVD3与包被的DHVD3竞争生物素标记的抗DHVD3单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，DHVD3浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中DHVD3的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的DHVD3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Serum and Urinary Soluble α-Klotho as Markers of Kidney and Vascular Impairment
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Exploring the beneficial effects and mechanisms of Cerasus humilis (Bge.) Sok fruit for calcium supplementation and promotion
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Association of serum 25-hydroxyvitamin D3, fibroblast growth factor-23, and C1q/tumor necrosis factor-related protein-3 with coronary artery calcification in nondialysis chronic kidney disease patients
IF: 3.000 -
Unraveling the interaction between lead and calcium in occupationally exposed males: an exploratory observation study
IF: 3.000Journal： INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH (2023)PMID：37889332Reactivity：HumanSample Type：Serum -
Effect of vitamin D metabolites and gene expression of vitamin D receptor, and 1-alpha-hydroxylase related to the sperm quality
IF: 1.700 -
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial
IF: 1.600Journal： Journal of General and Family Medicine (2023)DOI：10.1002/jgf2.606PMID：37261039Reactivity：HumanSample Type：serum -
Fucoidan Attenuated Kidney and Bone Damage Caused by CKD-MBD in Mice by Upregulating Klotho
IF: 0.700Journal： Pharmacognosy Magazine (2023)Reactivity：MouseSample Type：serum -
Diosmin, a citrus fruit-derived phlebotonic bioflavonoid protects rats from chronic kidney disease-induced loss of bone mass and strength without deteriorating the renal function
IF: 6.317 -
Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial
IF: 5.435
实验操作视频
