EPI(Epinephrine/Adrenaline) ELISA Kit
中文名称：肾上腺素(EPI)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用EPI抗原包被于酶标板上，实验时样品或标准品中的EPI与包被的EPI竞争生物素标记的抗EPI单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，EPI浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中EPI的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的EPI，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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High frequency deep transcranial magnetic stimulation acutely increases β-endorphins in obese humans
IF: 3.179 -
Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias
IF: 2.766 -
Effects of stocking density on stress responses and meat quality characteristics of lambs transported for 45 minutes or 3 hours
IF: 0.974Journal： SMALL RUMINANT RESEARCH (2018)Reactivity：SheepSample Type：serum -
Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery
IF: 0.545Journal： JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2018)PMID：29519176Reactivity：RabbitSample Type：Serum,Plasma -
Perivascular radiofrequency renal denervation lowers blood pressure and ameliorates cardiorenal fibrosis in spontaneously hypertensive rats
IF: 2.806 -
Intranasal Dexmedetomidine on Stress Hormones, Inflammatory Markers, and Postoperative Analgesia after Functional Endoscopic Sinus Surgery
IF: 3.236 -
A Novel Swine Model of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well to Renal Denervation
IF: 2.852Journal： AMERICAN JOURNAL OF HYPERTENSION (2015)PMID：25958303Reactivity：PigSample Type：Plasma,Urine -
Endurance training increases brain lactate uptake during hypoglycemia by up regulation of brain lactate transporters
IF: 4.241Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2014)PMID：25004253Reactivity：RatSample Type：plasma
实验操作视频
