FA/VB9(Folic Acid/Vitamin B9) ELISA Kit
中文名称：叶酸/维生素B9(FA/VB9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用FA/VB9抗原包被于酶标板上，实验时样品或标准品中的FA/VB9与包被的FA/VB9竞争生物素标记的抗FA/VB9单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，FA/VB9浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中FA/VB9的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的FA/VB9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism
IF: 12.200 -
Probiotic characteristics and whole genome sequence analysis of Lactiplantibacillus plantarum ZFM518 isolated from infant feces
IF: 7.400 -
Whole genome sequence analysis and in vitro probiotic characteristics of Lactococcus lactis ZFM559
IF: 6.000Journal： LWT-FOOD SCIENCE AND TECHNOLOGY (2024)Reactivity：Lactococcus lactis ZFM559 -
Betaine and B12 intake, glutathione concentration, and MTHFR, PEMT, and MTHFD1 genotypes are associated with diabetes-related parameters in Polish adults
IF: 3.700Journal： JOURNAL OF NUTRITION (2024)Reactivity：HumanSample Type：serum -
Potential probiotic characteristics and genomic analysis of a new folate-producing lactic acid bacteria Lactiplantibacillus plantarum ZFM55
IF: 3.300Journal： JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2024)Reactivity：Lactiplantibacillus plantarum -
Relationship between calcium-to-magnesium ratio and malaria parasite density among children with uncomplicated malaria infection
IF: 0.800 -
Mitochondrial folate pathway regulates myofibroblast differentiation and silica-induced pulmonary fibrosis
IF: 7.400 -
Mitochondrial DNA and Epigenetics: Investigating Interactions with the One-Carbon Metabolism in Obesity
IF: 7.310Journal： Oxidative Medicine and Cellular Longevity (2022)PMID：35132354Reactivity：HumanSample Type：Serum -
Comparison of serum B12, folate and homocysteine concentrations in children with autism spectrum disorder or attention deficit hyperactivity disorder and healthy controls
IF: 2.989Journal： Neuropsychiatric Disease and Treatment (2022)PMID：31496704Reactivity：HumanSample Type：Serum -
Metabolic Analysis of Methylenetetrahydrofolate Reductase Single Nucleotide Polymorphisms (MTHFR 677C
Journal： Indian Journal of Clinical Biochemistry (2022)PMID：37234187PMC10205924Reactivity：HumanSample Type：serum
Q1:E-EL-0009 FA/VB9是否可以检测饲料中的叶酸？
E-EL-0009 FA/VB9是通用型ELISA检测试剂盒，可以检测人、大鼠和小鼠的生物相关液体中叶酸含量。我们内部没有验证过饲料中的叶酸，但目前饲料中叶酸的含量检测还是微生物检测方法居多。
实验操作视频
