Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

一键复制产品信息

中文名称：游离脂肪酸（NEFA/FFA）荧光测定试剂盒

货号：E-BC-F039
文献（18）
说明书

说明书

Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

Price: ¥6850.00 ¥4180.00

Size:

96 T (80 samples) 48 T (32 samples)

样本类型： 血清（浆）;动物组织;细胞
检测仪器及波长： 荧光酶标仪（激发波长为535 nm，发射波长为590 nm）
研究领域： 代谢疾病;糖脂代谢;能量代谢

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	游离脂肪酸在酰基合成酶的作用下生成酰基辅酶A，酰基辅酶A在酰基氧化酶的氧化作用下生成过氧化氢。过氧化氢在酶和探针的存在下反应，产生的荧光物质在激发波长535 nm和发射波长590 nm处的荧光强度与游离脂肪酸浓度成正比。
指标简称	NEFA
灵敏度	1.20 μmol/L
检测范围	1.20-100 μmol/L
精密度	批间差：5.1% \| 批内差：4.1%
样本量	50 μL
检测时间	1 h 10 min
检测仪器及波长	荧光酶标仪（激发波长为535 nm，发射波长为590 nm）
保存条件	该产品可在-20°C避光条件下保存6个月。
有效期	6个月

Zhuyu pill attenuates metabolic-associated fatty liver disease by regulating macrophage polarization through TLR4 signaling pathway

IF: 6.700

Journal： PHYTOMEDICINE (2025)

DOI：10.1016/j.phymed.2025.156439

Reactivity：Mouse

Sample Type：liver tissue
Structural characterisation and hypoglycaemic effects on type 2 diabetic mice of Spirulina platensis polysaccharides and Se-modified polysaccharides

IF: 4.800

Journal： Food Bioscience (2025)

DOI：10.1016/j.fbio.2025.105826

Reactivity：Mouse

Sample Type：serum
Ablation of Hepatic Asah1 Gene Disrupts Hepatic Lipid Homeostasis and Promotes Fibrotic Non-Alcoholic Steatohepatitis in Mice

IF: 4.700

Journal： AMERICAN JOURNAL OF PATHOLOGY (2024)

DOI：10.1016/j.ajpath.2024.11.003

Reactivity：Mouse

Sample Type：plasma
Integrating untargeted and oxylipins-targeted metabolomics to reveal the anti-obesity and hypolipidemic mechanism of conjugated linoleic acid in high-fat diet rats

IF: 3.800

Journal： Journal of Functional Foods (2024)

DOI：10.1016/j.jff.2024.106182

Reactivity：Rat

Sample Type：serum
Novel Dual PPAR δ/γ Partial Agonist Induces Hepatic Lipid Accumulation through Direct Binding and Inhibition of AKT1 Phosphorylation, Mediating CD36 Upregulation

IF: 3.700

Journal： CHEMICAL RESEARCH IN TOXICOLOGY (2024)

DOI：10.1021/acs.chemrestox.4c00268

Reactivity：Mouse

Sample Type：Liver tissue
Isoliquiritigenin Prevents the Development of Nephropathy by an HFD in Rats Through the Induction of Antioxidant Production and Inhibition of the MD-2/TLR4/NF-κB Pathway

IF: 3.600

Journal： Biology-Basel (2024)

DOI：10.3390/biology13120984

Reactivity：Rat

Sample Type：serum,kidney tissue
Dictamnine Reduces Gestational Diabetic Complications in Streptozotocin-Induced Diabetic Pregnant Rats via Regulating Inflammation and NOX-2, MCP-1/AGE–RAGE Pathway

IF: 1.400

Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)

DOI：10.1007/s43450-024-00591-y

Reactivity：Rat

Sample Type：serum
Dihydrosphingolipids are associated with steatosis and increased fibrosis damage in non-alcoholic fatty liver disease

IF: 4.800

Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS (2023)

DOI：10.1016/j.bbalip.2023.159318

PMID：37059386

Reactivity：Mouse

Sample Type：plasma,liver tissue
Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

IF: 17.694

Journal： Nature Communications (2022)

DOI：10.1038/s41467-022-35481-1

PMID：36572670

Reactivity：Mouse

Sample Type：serum
Combined effect of polystyrene microplastics and bisphenol A on the human embryonic stem cells-derived liver organoids: The hepatotoxicity and lipid accumulation

IF: 10.753

Journal： SCIENCE OF THE TOTAL ENVIRONMENT (2022)

DOI：10.1016/j.scitotenv.2022.158585

PMID：36089014

Reactivity：Human

E-BC-K013-M	Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit	96 T (40 samples) , 48 T (16 samples)
E-BC-K013-S	Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit	100 Assays (50 samples) , 50 Assays (17 samples)