fT3(Free Triiodothyronine) ELISA Kit
中文名称：游离三碘甲状腺原氨酸(fT3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用fT3抗原包被于酶标板上，实验时样品或标准品中的fT3与包被的fT3竞争生物素标记的抗fT3单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，fT3浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中fT3的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的fT3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Variation of Photoperiod Regime during the American Bullfrog Tadpole Stage: Influence on Growth and Metamorphosis
IF: 1.717Journal： NORTH AMERICAN JOURNAL OF AQUACULTURE (2021)Reactivity：FrogSample Type：tadpole -
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors
IF: 6.018Journal： Computational and Structural Biotechnology Journal (2020)PMID：33304464Reactivity：RatSample Type：serum -
Effects of butylparaben exposure on thyroid peroxidase (TPO) and type 1 iodothyronine deiodinase (D1) in female Wistar rats
IF: 4.099 -
Effect of short-term and long-term traffic noise exposure on the thyroid gland in adult rats: a sexual dimorphic study
Journal： Hormone Molecular Biology and Clinical Investigation (2020)PMID：33781004Reactivity：RatSample Type：plasma -
Unraveling the toxic effects of neonicotinoid insecticides on the thyroid endocrine system of lizards
IF: 5.714 -
Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway
IF: 4.527Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019)PMID：30771657Reactivity：MouseSample Type：Plasma -
Effect of quercetin supplementation on the growth, feed efficiency and serum hormone levels of New Zealand White rabbits
IF: 0.653Journal： SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCE (2019)Reactivity：RabbitSample Type：serum -
The body burden and thyroid disruption in lizards (Eremias argus) living in benzoylurea pesticides-contaminated soil
IF: 6.434 -
Dose-dependent effects of flufenoxuron on thyroid system of mature female lizards (Eremias argus) and their offspring
IF: 4.610 -
Metabolism of Diflubenzuron in Lizard (Eremias argus) and Comparative Toxicity of Diflubenzuron and Its Metabolite
IF: 3.412Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2018)PMID：30346759Reactivity：LizardSample Type：Serum
实验操作视频
