fT3(Free Triiodothyronine) ELISA Kit
中文名称：游离三碘甲状腺原氨酸(fT3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用fT3抗原包被于酶标板上，实验时样品或标准品中的fT3与包被的fT3竞争生物素标记的抗fT3单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，fT3浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中fT3的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的fT3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of central FGF21 infusion on the hypothalamus–pituitary–thyroid axis and energy metabolism in rats
IF: 2.757 -
Growth and male reproduction improvement of non-thermal dielectric barrier discharge plasma treatment on chickens
IF: 2.373Journal： JOURNAL OF PHYSICS D-APPLIED PHYSICS (2018)Reactivity：ChickenSample Type：Serum -
Central irisin administration suppresses thyroid hormone production but increases energy consumption in rats
IF: 2.159 -
Stereoselective degradation and thyroid endocrine disruption of lambda-cyhalothrin in lizards (Eremias argus) following oral exposure
IF: 5.099 -
Oral and dermal diflubenzuron exposure causes a hypothalamic–pituitary–thyroid (HPT) axis disturbance in the Mongolian racerunner (Eremias argus)
IF: 5.099 -
Unraveling the different toxic effect of flufenoxuron on the thyroid endocrine system of the Mongolia racerunner (Eremias Argus) at different stages
IF: 4.208 -
Fenugreek, A Potent Hypoglycaemic Herb Can Cause Central Hypothyroidism Via Leptin – A Threat To Diabetes Phytotherapy
IF: 1.685Journal： EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2017)PMID：28407664Reactivity：RatSample Type：Serum -
Effects of Thyroid Hormone Therapy on Cut-Surface Healing of the Remnant Stomach with Short-Term Weight Loss Alterations after Sleeve Gastrectomy
IF: 1.532Journal： JOURNAL OF INVESTIGATIVE SURGERY (2017)PMID：28635514Reactivity：RatSample Type：Serum,Tissue homogenate -
Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteins
IF: 0.904Journal： International Journal of Peptide Research and Therapeutics (2017)Reactivity：RatSample Type：Serum
实验操作视频
