GnRH(Gonadotropin Releasing Hormone) ELISA Kit
中文名称：促性腺激素释放激素(GnRH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用GnRH抗原包被于酶标板上，实验时样品或标准品中的GnRH与包被的GnRH竞争生物素标记的抗GnRH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，GnRH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中GnRH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的GnRH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice
IF: 8.400Journal： Cell Communication and Signaling (2024)PMID：38491517Reactivity：MouseSample Type：plasma,prostate tissue -
The Effect of Thymoquinone on the TNF-α/OTULIN/NF-κB Axis Against Cisplatin-?nduced Testicular Tissue Damage
IF: 2.600Journal： Reproductive Sciences (2024)Reactivity：RatSample Type：serum -
Combination Therapy with Vitamin D and Metformin: A Potential Approach to Mitigate Testicular Dysfunction in Type 2 Diabetes Mellitus
IF: 2.600Journal： Reproductive Sciences (2024)Reactivity：RatSample Type：Serum -
The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period
IF: 2.500Journal： Journal of Integrative Neuroscience (2024)Reactivity：MouseSample Type：Serum -
Prenatal cadmium exposure has intergenerational adverse effects on the Sertoli cells through FSHR pathway
IF: 3.800 -
Relaxin (a regulatory-peptide) enhances cryotolerance, fertility potential and plasma reproductive hormones of Nili Ravi buffalo (Bubalus bubalis) during low breeding season
IF: 2.400 -
Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway
IF: 7.666 -
Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway
IF: 7.419 -
Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway
IF: 7.033Journal： International Journal of Nanomedicine (2022)PMID：35401001Reactivity：MouseSample Type：serum -
Studies on testicular ultrastructural and hormonal changes in type-2 diabetic rats treated with highly active antiretroviral therapy conjugated silver nanoparticles
IF: 6.780
实验操作视频
