GSH(Glutathione) ELISA Kit
中文名称：谷胱甘肽(GSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 μg/mL
- 灵敏度： 0.94 μg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用GSH抗原包被于酶标板上，实验时样品或标准品中的GSH与包被的GSH竞争生物素标记的抗GSH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，GSH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中GSH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 μg/mL |
| 灵敏度 | 0.94 μg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的GSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Comprehensive dry eye therapy: overcoming ocular surface barrier and combating inflammation, oxidation, and mitochondrial damage
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METTL14 depletion ameliorates ferroptosis in severe acute pancreatitis by increasing the N6-methyladenosine modification of ACSL4 and STA1
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Borax induces ferroptosis of glioblastoma by targeting HSPA5/NRF2/GPx4/GSH pathways
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Age-Related Effects of AT1 Receptor Antagonist Losartan on Cognitive Decline in Spontaneously Hypertensive Rats
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Costunolide Inhibits Chronic Kidney Disease Development by Attenuating IKKβ/NF-κB Pathway
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Tomatidine Alleviates Intervertebral Disc Degeneration by Activating the Nrf2/HO-1/GPX4 Signaling Pathway
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Humanin Attenuates Metabolic, Toxic, and Traumatic Neuropathic Pain in Mice by Protecting Against Oxidative Stress and Increasing Inflammatory Cytokine
IF: 4.600Journal： NEUROPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum
实验操作视频
