GSH(Glutathione) ELISA Kit
中文名称：谷胱甘肽(GSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 μg/mL
- 灵敏度： 0.94 μg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用GSH抗原包被于酶标板上，实验时样品或标准品中的GSH与包被的GSH竞争生物素标记的抗GSH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，GSH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中GSH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 μg/mL |
| 灵敏度 | 0.94 μg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的GSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Limonin modulated immune and inflammatory responses to suppress colorectal adenocarcinoma in mice model
IF: 3.000Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2021)PMID：34009457Reactivity：MouseSample Type：Serum -
Dehydroabietic acid improves nonalcoholic fatty liver disease through activating the Keap1/Nrf2-ARE signaling pathway to reduce ferroptosis
IF: 2.343Journal： Journal of Natural Medicines (2021)PMID：33590347Reactivity：MouseSample Type：Tissue homogenate(liver) -
Effects of shikonin on the development of ovarian follicles and female germline stem cells.
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Stimuli-Responsive Polysaccharide Enveloped Liposome for Targeting and Penetrating Delivery of survivin-shRNA into Breast Tumor
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Reproductive effects of subchronic exposure to acetamiprid in male rats
IF: 3.998Journal： Scientific Reports (2020)PMID：32488017Reactivity：RatSample Type：Plasma,Tissue homogenate -
Perindopril ameliorates experimental Alzheimer’s disease progression: role of amyloid β degradation, central estrogen receptor and hyperlipidemic-lipid raft signaling
IF: 3.238Journal： INFLAMMOPHARMACOLOGY (2020)Reactivity：RatSample Type：Serum,Tissue homogenate -
Metformin attenuated histopathological ocular deteriorations in a streptozotocin-induced hyperglycemic rat model
IF: 2.050Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020)PMID：33047165Reactivity：RatSample Type：Serum -
Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro
IF: 1.951 -
The hepato-protective effect of eupatilin on an alcoholic liver disease model of rats
IF: 1.805Journal： KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY (2020)Reactivity：RatSample Type：plasma -
Myrothamnus flabellifolius attenuates streptozotocin-high energy diet-induced type 2 diabetes in male sprague dawley rats
Journal： Journal of Medicinal Plants Research (2020)Reactivity：RatSample Type：Plasma
实验操作视频
