GSH(Glutathione) ELISA Kit
中文名称：谷胱甘肽(GSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 μg/mL
- 灵敏度： 0.94 μg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用GSH抗原包被于酶标板上，实验时样品或标准品中的GSH与包被的GSH竞争生物素标记的抗GSH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，GSH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中GSH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 μg/mL |
| 灵敏度 | 0.94 μg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的GSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Niacin modulates depressive-like behavior in experimental colitis through GPR109A-dependent mechanisms
IF: 6.1 -
Protective effects of harmine on Monosodium Iodoacetate-induced Osteoarthritis in rats: In vitro and in vivo studies
IF: 6.000Journal： Arabian Journal of Chemistry (2023)Reactivity：RatSample Type：joint tissues -
Comparative Analysis of Anticonvulsant Activity of Trans and Cis 5,5′-Diphenylhydantoin Schiff Bases
IF: 5.6Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：38003260Reactivity：MouseSample Type：hippocampi -
Cancer-associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1
IF: 5.500 -
Curculigoside mitigates dextran sulfate sodium?induced colitis by activation of KEAP1?NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier
IF: 5.4Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2023)PMID：37772380Reactivity：Mouse，HumanSample Type：colonic tissue -
Characterization of Sesuvium sesuvioides Using High-Performance Liquid Chromatography and Validation of Its In Vivo Anti-hyperthyroidism Effect via Suppressing Oxidative Stress and Inflammatory Markers
IF: 4.1 -
Chlorogenic Acid Attenuates Doxorubicin-Induced Oxidative Stress and Markers of Apoptosis in Cardiomyocytes via Nrf2/HO-1 and Dityrosine Signaling
IF: 3.508Journal： Journal of Personalized Medicine (2023)PMID：37109035Reactivity：RatSample Type：cardiac tissue -
Protective effects of chronic humanin treatment in mice with diabetic encephalopathy: A focus on oxidative stress, inflammation, and apoptosis
IF: 2.7Journal： BEHAVIOURAL BRAIN RESEARCH (2023)PMID：37467966Reactivity：MouseSample Type：prefrontal cortex,hippocampus -
N-acetylcysteine ameliorates chemotherapy-induced impaired anxiety and depression-like behaviors by regulating inflammation, oxidative and cholinergic status, and BDNF release.
IF: 2.7Journal： BEHAVIOURAL BRAIN RESEARCH (2023)PMID：37926333Reactivity：RatSample Type：serum,hippocampal -
Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria
IF: 12.822
实验操作视频
