High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)
中文名称:高密度脂蛋白胆固醇(HDL-C)比色法测试盒(双试剂直接法)

Price:
Size:
96 T (92 samples)
- 样本类型: 血清(浆)
- 检测仪器及波长: 酶标仪(530-570 nm,最佳检测波长为546 nm)
- 研究领域: 代谢疾病;糖脂代谢
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
CM、VLD L 、LDL 在多聚阴离子环境下发生凝聚生成聚合物并被多聚体掩蔽, 高密度脂蛋白(HDL)在表面活性剂的作用下形成可溶性复合物,从而使 HDL-C 可直接与酶试剂[含胆固醇酯酶(CE )、胆固醇氧化酶 ( CO ) 等]发生反应,产生过氧化氢,过氧化氢在 4-氨基安替吡啉(4-AA)和酚(TOOS)存在时,经过氧化物酶(peroxidase,POD)催化,反应生成苯醌亚胺非那腙的红色醌类化物,其颜色深浅与 HDL-C 含量成正比。
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指标简称 | HDL-C |
灵敏度 | 0.06 mmol/L |
检测范围 | 0.06-3.8 mmol/L |
精密度 | 批间差:5% | 批内差:3% |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(530-570 nm,最佳检测波长为546 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 12个月 |
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Q1:样本中含有表面活性剂SDS对检测有影响吗?
会对酶试剂有影响,从而影响检测结果。

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