High Sensitivity Human IL-1β (Interleukin 1 Beta) ELISA Kit
中文名称：高敏型人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.63-40 pg/mL
- 灵敏度： 0.39 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-1β浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.63-40 pg/mL |
| 灵敏度 | 0.39 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Cinobufagin inhibits M2?like tumor?associated macrophage polarization to attenuate the invasion and migration of lung cancer cells
IF: 4.5 -
Rehydration effect of qingshu buye decoction on exercise and high temperature-induced dehydration
IF: 4.5Journal： Journal of the International Society of Sports Nutrition (2024)Reactivity：HumanSample Type：serum -
SCG2 mediates blood–brain barrier dysfunction and schizophrenia-like behaviors after traumatic brain injury
IF: 4.4 -
The mitochondrial and cytoplasmic superoxide anion imbalance trigger the expression of certain cellular aging markers in HaCaT keratinocytes
IF: 4.4 -
Isoliquiritin counteracts cadmium-induced intestinal damage in mice through enhancing intestinal barrier function and inhibiting apoptosis
IF: 4.3 -
Green synthesis of Ag nanoparticles from Verbascum insulare Boiss. and Heldr.: Evaluation of antimicrobial, anticancer, antioxidant properties and photocatalytic degradation of MB
IF: 4.3Journal： JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY (2024)Sample Type：A549 cell -
Potential Molecular Mechanism of Illicium simonsii Maxim Petroleum Ether Fraction in the Treatment of Hepatocellular Carcinoma
IF: 4.3 -
Antioxidant and Anti-Inflammatory Effects of Nettle Polyphenolic Extract: Impact on Human Colon Cells and Cytotoxicity Against Colorectal Adenocarcinoma
IF: 4.2 -
Ultra-Performance Liquid Chromatography and Mass Spectrometry Characterization, and Antioxidant, Protective, and Anti-Inflammatory Activity, of the Polyphenolic Fraction from Ocimum basilicum
IF: 4.2 -
Licochalcone A Ameliorates Aspergillus fumigatus Keratitis by Reducing Fungal Load and Activating the Nrf2/HO-1 Signaling Pathway
IF: 4.0
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
