High Sensitivity Human IL-1β (Interleukin 1 Beta) ELISA Kit
中文名称：高敏型人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.63-40 pg/mL
- 灵敏度： 0.39 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-1β浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.63-40 pg/mL |
| 灵敏度 | 0.39 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Correlation of serum Orexin-A level with cognitive function and serum inflammatory cytokines in epileptic patients
IF: 2.2Journal： American Journal of Translational Research (2023)DOI：PMID:37434836PMID：37434836Reactivity：HumanSample Type：serum -
The effect of secukinumab treatment for psoriasis on serum cytokines and correlation with disease severity
IF: 2.2 -
Upregulation of HTRA1 mediated by the lncRNA NEAT1/miR-141-3p axis contributes to endometriosis development through activating NLRP3 inflammasome-mediated pyroptotic cell death and cellular inflammation
IF: 2.100Journal： IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2023)PMID：37017808Reactivity：HumanSample Type：Endometrial tissue -
Relationship between miRNA-21, miRNA-155, and miRNA-182 expression and inflammatory factors in cerebrospinal fluid from patients with multiple sclerosis
IF: 1.9Journal： CLINICAL NEUROLOGY AND NEUROSURGERY (2023)PMID：37453285Reactivity：HumanSample Type：cerebrospinal fluid (CSF) -
Effect of mesencephalic astrocyte-derived neurotrophic factor on the inflammatory response in human gingival fibroblasts cells
IF: 1.9 -
The effect of heart failure on gut microbial richness and diversity
IF: 1.800 -
Biochemical and gene expression studies reveal the potential of Aspergillus oryzae-fermented broken rice and brewers' rice water extracts as anti-photoageing agents
IF: 1.6 -
TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction
IF: 1.200 -
Effect of Fetuin-A and oxidative stress on the occurrence of unexplained infertility
IF: 1.1Journal： Turkish Journal of Obstetrics and Gynecology (2023)PMID：37260171Reactivity：HumanSample Type：serum -
Role and significance of SIRT1 in regulating the LPS-activated pyroptosis pathway in children with congenital hydronephrosis
IF: 0.8
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
