High Sensitivity Mouse IL-1β (Interleukin 1 Beta) ELISA Kit
中文名称：高敏型小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.41 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-1β浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.41 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Gentiopicroside abrogates lipopolysaccharide-induced depressive-like behavior in mice through tryptophan-degrading pathway
IF: 2.441 -
Dioscin prevents LPS?induced acute lung injury through inhibiting the TLR4/MyD88 signaling pathway via upregulation of HSP70
IF: 1.922Journal： Molecular Medicine Reports (2018)PMID：29512786Reactivity：MouseSample Type：Serum,Tissue homogenate -
Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses
IF: 1.922 -
Embelin inhibits abdominal aortic aneurysm through decreasing IL?6?induced STAT3 and NF?κB inactivation
IF: 1.922Journal： Molecular Medicine Reports (2018)PMID：29956759Reactivity：MouseSample Type：Aortic tissues -
The association between inflammation, the microbiome and urethane-induced pulmonary adenocarcinoma Corrigendum in /10.3892/ol.2018.9063
IF: 1.664 -
NLRP3 inflammasome signaling as an early molecular response is negatively controlled by miR-186 in CFA-induced prosopalgia mice
IF: 1.492Journal： BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2018)PMID：30020320Reactivity：MouseSample Type：Serum,Tissue homogenate -
miR?148a inhibits pro?inflammatory cytokines released by intervertebral disc cells by regulating the p38/MAPK pathway
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30186499Reactivity：HumanSample Type：Cell culture supernatant -
Gentiopicroside protects neurons from astrocyte-mediated inflammatory injuries by inhibition of nuclear factor-κB and mitogen-activated protein kinase signaling pathways
IF: 1.266 -
Celastrol-Induced Nur77 Interaction with TRAF2 Alleviates Inflammation by Promoting Mitochondrial Ubiquitination and Autophagy
IF: 14.714 -
A novel mechanism of diabetic vascular endothelial dysfunction: Hypoadiponectinemia-induced NLRP3 inflammasome activation
IF: 5.476Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2017)PMID：28216285Reactivity：MouseSample Type：Serum,Cell culture supernatant
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