High Sensitivity Mouse IL-1β (Interleukin 1 Beta) ELISA Kit
中文名称：高敏型小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.41 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-1β浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.41 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Lactobacillus plantarum 17-1 Ameliorates DSS-Induced Colitis by Modulating the Colonic Microbiota Composition and Metabolome in Mice
IF: 4.8 -
PINK1 affects mitochondrial oxidative phosphorylation by regulating MFN2 to alleviate diabetic kidney disease
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：serum -
Zhi-Zi-Chi decoction ameliorates depression-like behavior in chronic unpredictable mild stress-induced mice via the PI3K/AKT/mTOR signaling pathway
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：hippocampi tissue -
Epimedii Folium and Curculiginis Rhizoma ameliorate age-related cognitive decline and neuroinflammation through modulation of the NLRP3 inflammasome
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：serum -
Therapeutic effects of Saussurea graminea Dunn and its active compounds in Sepsis-associated liver injury: Transcriptomics, metabolomics and experimental validation
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：serum -
Overexpression of TGFBR3 Aggravates Cognitive Impairment and Neuroinflammation by Promoting Microglia M1 Polarization in the APP/PS1 Mouse Model of Alzheimer's Disease
IF: 4.6Journal： MOLECULAR NEUROBIOLOGY (2025)Reactivity：MouseSample Type：serum -
TREM2 Modulates Postoperative Cognitive Function in Aged Mice by Inhibiting the NLRP3/caspase-1 Pathway and Apoptosis via PLCγ2 Activation
IF: 4.6Journal： MOLECULAR NEUROBIOLOGY (2025)Reactivity：MouseSample Type：Hippocampal tissue -
ACVR1 Drives Neuropathic Pain by Regulating NLRP3-Induced Neuronal Pyroptosis through the p38 and Smad1/5/8 Pathways
IF: 4.6Journal： NEUROPHARMACOLOGY (2025)Reactivity：MouseSample Type：Serum -
MiR-425-5p intervenes in autoimmune myocarditis by regulating Treg cell differentiation through NRAS
IF: 4.6Journal： Frontiers in Cell and Developmental Biology (2025)Reactivity：MouseSample Type：serum -
Uridine Phosphorylase 1 as a Biomarker Associated with Glycolysis in Acute Lung Injury
IF: 4.5Journal： INFLAMMATION (2025)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF)
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
