High Sensitivity Mouse IL-1β (Interleukin 1 Beta) ELISA Kit

中文名称：高敏型小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒

货号：E-HSEL-M0001
文献（1134）
说明书

说明书

别称：

Il1b、Interleukin-1 beta、IL-1 beta

High Sensitivity Mouse IL-1β (Interleukin 1 Beta) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 3.13-200 pg/mL
灵敏度： 1.41 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-1β浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	3.13-200 pg/mL
灵敏度	1.41 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Collagen peptide and taurine synergistically improve hypertensive kidney injury induced by high salt diet through gut-kidney axis

IF: 4.8

Journal： Food Bioscience (2024)

DOI：10.1016/j.fbio.2024.105320

Reactivity：Mouse

Sample Type：kidney tissue,jejunum tissue
Codonopsis pilosula polysaccharide alleviates ulcerative colitis by modulating gut microbiota and SCFA/GPR/NLRP3 pathway

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)

DOI：10.1016/j.jep.2024.118928

Reactivity：Mouse

Sample Type：colon tissue
ATP citrate lyase ablation hampers exocrine regeneration via TLR4/NF-kappaB signaling after acute pancreatitis in mice

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.113485

Reactivity：Mouse

Sample Type：Plasma
Protective Effects of Isostrictiniin Against High-Fat, High-Sugar Diet-Induced Steatosis in MASLD Mice via Regulation of the AMPK/SREBP-1c/ACC Pathway

IF: 4.8

Journal： Nutrients (2024)

DOI：10.3390/nu16223876

Reactivity：Mouse

Sample Type：liver tissue
Relationship Between Metabolomics of T2DM Patients and the Anti-Diabetic Effects of Phellodendri Chinensis Cortex-Anemarrhenae Rhizoma Herb Pair in Mice

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)

DOI：10.1016/j.jep.2024.119129

Reactivity：Mouse

Sample Type：serum
Network pharmacology-based strategy to reveal Acacetin against lipopolysaccharide-induced lung injury

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.113843

Reactivity：Mouse

Sample Type：lung tissue
Novel insights into cuproptosis in alcoholic liver disease using bioinformatics analysis and experimental validation

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.113828

Reactivity：Mouse

Sample Type：Serum
Taraxasterol extracted from Ixeridium gramineum (Fisch.) Tzvel. attenuated D-GalN/LPS-Induced Fulminant Hepatitis by modulating the JAK/STAT and TNF signalling pathways

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)

DOI：10.1016/j.jep.2024.119256

Reactivity：Mouse

Sample Type：liver tissue
Formononetin Alleviates Ischemic Acute Kidney Injury by Regulating Macrophage Polarization through KLF6/STAT3 Pathway

IF: 4.8

Journal： AMERICAN JOURNAL OF CHINESE MEDICINE (2024)

DOI：10.1142/S0192415X24500587

Reactivity：Mouse

Sample Type：serum
Uncovering the pharmacological mechanisms of Patchouli essential oil for treating ulcerative colitis

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)

DOI：10.1016/j.jep.2024.118737

Reactivity：Mouse

Sample Type：colon tissue

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