High Sensitivity Mouse IL-6 (Interleukin 6) ELISA Kit
中文名称：高敏型小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.78-50 pg/mL
- 灵敏度： 0.49 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 pg/mL |
| 灵敏度 | 0.49 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Demethylenetetrahydroberberine alleviates nonalcoholic fatty liver disease by inhibiting the NLRP3 inflammasome and oxidative stress in mice
IF: 5.037 -
Pancreastatin induces hepatic steatosis in type 2 diabetes by impeding mitochondrial functioning
IF: 5.037 -
Empagliflozin, a sodium glucose cotransporter-2 inhibitor, ameliorates peritoneal fibrosis via suppressing TGF-β/Smad signaling
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33517222Reactivity：MouseSample Type：peritoneal dialysis solution,Cell culture supernatant -
Hydrogen gas alleviates sepsis-induced neuroinflammation and cognitive impairment through regulation of DNMT1 and DNMT3a-mediated BDNF promoter IV methylation in mice
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33773206Reactivity：MouseSample Type：Tissue homogenate(hippocampal tissues) -
Protective effect of combination of anakinra and MCC950 against acute lung injury is achieved through suppression of the NF-κB-mediated-MAPK and NLRP3-caspase pathways
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：34022766Reactivity：MouseSample Type：Tissue homogenate(lung),Bronchoalveolar Lavage Fluid -
Oxymatrine inhibits neuroinflammation by?Regulating M1/M2 polarization in N9 microglia through the TLR4/NF-κB pathway
IF: 4.932 -
Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway
IF: 4.932 -
Senescence marker protein 30 (SMP30) protects against high glucose-induced apoptosis, oxidative stress and inflammatory response in retinal ganglion cells by enhancing Nrf2 activation via regulation of Akt/GSK-3β pathway
IF: 4.932 -
Artesunate attenuates atherosclerosis by inhibiting macrophage M1-like polarization and improving metabolism
IF: 4.932 -
The Role of Indoleamine 2,3-Dioxygenase in Renal Tubular Epithelial Cells Senescence under Anoxia or Reoxygenation
IF: 4.879
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