High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-M0009
文献（1043）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 4.69-300 pg/mL
灵敏度： 2.76 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	4.69-300 pg/mL
灵敏度	2.76 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

RBM15 promotes m6A methylation and stability of KLF6 mRNA to accelerate pyroptosis of retinal ganglion cells in early-stage diabetic retinopathy

IF: 2.2

Journal： JOURNAL OF MOLECULAR HISTOLOGY (2025)

DOI：10.1007/s10735-025-10458-8

Reactivity：Mouse

Sample Type：serum
Ophiopogonin D Alleviates Sepsis-Induced Acute Lung Injury Through Improving Microvascular Endothelial Barrier Dysfunction via Inhibition of HIF-1α-VEGF Pathway

IF: 1.8

Journal： CELL BIOCHEMISTRY AND BIOPHYSICS (2025)

DOI：10.1007/s12013-024-01661-7

Reactivity：Mouse

Sample Type：lung tissue,bronchoalveolar lavage fluid (BALF),serum
NINJ1 impairs the anti-inflammatory function of hUC-MSCs with synergistic IFN-γ and TNF-α stimulation

IF: 1.8

Journal： Chinese Journal of Traumatology (2025)

DOI：10.1016/j.cjtee.2025.04.003

Reactivity：Human,Mouse
In vitro anti-inflammatory and in silico anti-viral assessment of phytoconstituents in polyherbal Ayurvedic formulation ‘Arogyamrita Kwath’

IF: 1.7

Journal： Journal of Ayurveda and Integrative Medicine (2025)

DOI：10.1016/j.jaim.2024.101076

Reactivity：Mouse
Upregulation of miR-22-3p Alleviates Hyperalgesia and Neuroinflammation Caused by Migraine

IF: 1.6

Journal： SYNAPSE (2025)

DOI：10.1002/syn.70017

Reactivity：Mouse

Sample Type：trigeminal nucleus caudalis (TNC) tissue
Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model

IF: 1.6

Journal： Iranian Journal of Medical Sciences (2025)

DOI：10.30476/ijms.2024.102396.3530

Reactivity：Mouse

Sample Type：colon tumor tissue
Aucubin Regulates Allergic Airway Inflammation in Ovalbumin-induced Allergic Asthma in Mice by Controlling Th2 Cytokines

IF: 0.6

Journal： Pharmacognosy Magazine (2025)

DOI：10.1177/09731296241305864

Reactivity：Mouse

Sample Type：Bronchoalveolar Lavage Fluid (BALF)
Acetate enables metabolic fitness and cognitive performance during sleep disruption

IF: 27.7

Journal： Cell Metabolism (2024)

DOI：10.1016/j.cmet.2024.07.019

Reactivity：Mouse

Sample Type：hypothalamus tissue
Dietary-timing-induced gut microbiota diurnal oscillations modulate inflammatory rhythms in rheumatoid arthritis

IF: 27.7

Journal： Cell Metabolism (2024)

DOI：10.1016/j.cmet.2024.08.007

Reactivity：Mouse

Sample Type：serum
A Customized Biohybrid presenting Cascade Responses to Tumor Microenvironment

IF: 27.4

Journal： ADVANCED MATERIALS (2024)

DOI：10.1002/adma.202404901

Reactivity：Mouse

Sample Type：serum

Species

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