High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-M0009
文献（1043）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 4.69-300 pg/mL
灵敏度： 2.76 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	4.69-300 pg/mL
灵敏度	2.76 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Ginsenoside Rb1-loaded bionic nanoparticles alleviate sepsis-induced acute lung injury by reducing mitochondrial oxidative stress to inhibit macrophage PANoptosis

IF: 7.6

Journal： MATERIALS & DESIGN (2024)

DOI：10.1016/j.matdes.2024.113291

Reactivity：Mouse

Sample Type：bronchoalveolar lavage fluid (BALF)
Engineering an energy-dissipating hybrid tissue in?vivo for obesity treatment

IF: 7.5

Journal： Cell Reports (2024)

DOI：10.1016/j.celrep.2024.114425

Reactivity：Mouse

Sample Type：serum
Nuclear HMGB1 is critical for CD8 T?cell IFN-γ production and anti-tumor immunity

IF: 7.5

Journal： Cell Reports (2024)

DOI：10.1016/j.celrep.2024.114591

Reactivity：Mouse
Gut microbiota dysbiosis and oxidative damage in high-fat diet-induced impairment of spermatogenesis: Role of protocatechuic acid intervention

IF: 7.4

Journal： Food Frontiers (2024)

DOI：10.1002/fft2.484

Reactivity：Mouse

Sample Type：intestine tissue
Role of miR-21-5p in the protective effects of PLGA-encapsulated extracellular vesicles from urine-derived stem cells on periprosthetic osteolysis

IF: 7.2

Journal： Applied Materials Today (2024)

DOI：10.1016/j.apmt.2024.102205

Reactivity：Mouse
Sevoflurane exposure accelerates the onset of cognitive impairment via promoting p-Drp1S616-mediated mitochondrial fission in a mouse model of Alzheimer's disease

IF: 7.1

Journal： FREE RADICAL BIOLOGY AND MEDICINE (2024)

DOI：10.1016/j.freeradbiomed.2024.10.301

Reactivity：Mouse

Sample Type：hippocampal tissue
Potential structure–function relationships of pectic polysaccharides from quinoa microgreens: Impact of various esterification degrees

IF: 7.0

Journal： FOOD RESEARCH INTERNATIONAL (2024)

DOI：10.1016/j.foodres.2024.114395

Reactivity：Mouse
Indoxyl sulphate-TNFα axis mediates uremic encephalopathy in rodent acute kidney injury

IF: 6.9

Journal： ACTA PHARMACOLOGICA SINICA (2024)

DOI：10.1038/s41401-024-01251-6

Reactivity：Mouse

Sample Type：brain tissue
PEA-OXA restores cognitive impairments associated with vitamin D deficiency-dependent alterations of the gut microbiota

IF: 6.9

Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)

DOI：10.1016/j.biopha.2024.116600

Reactivity：Mouse

Sample Type：hippocampal tissue
THBru attenuates diabetic cardiomyopathy by inhibiting RAGE-dependent inflammation

IF: 6.9

Journal： ACTA PHARMACOLOGICA SINICA (2024)

DOI：10.1038/s41401-024-01307-7

Reactivity：Mouse

Sample Type：plasma

Species

{{relatedElisaPage.numTotal}} Results

All

Canine

Human

Mouse

Porcine

Rat

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual

Cat.No.：{{item.cat}}

{{index}}{{goods_show_value}}