High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-M0009
文献（1043）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 4.69-300 pg/mL
灵敏度： 2.76 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	4.69-300 pg/mL
灵敏度	2.76 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Green tea polyphenol alleviates silica particle-induced lung injury by suppressing IL-17/NF-κB p65 signaling-driven inflammation

IF: 6.7

Journal： PHYTOMEDICINE (2024)

DOI：10.1016/j.phymed.2024.156238

Reactivity：Mouse

Sample Type：Serum
Cmtm4 deficiency exacerbates colitis by inducing gut dysbiosis and S100A8/9 expression

IF: 6.6

Journal： Journal of Genetics and Genomics (2024)

DOI：10.1016/j.jgg.2024.03.009

Reactivity：Mouse

Sample Type：colon tissue
Mesoporous Polydopamine Nanotherapeutics for MRI-Guided Cancer Photothermal and Anti-Inflammatory Therapy

IF: 6.6

Journal： International Journal of Nanomedicine (2024)

DOI：10.2147/IJN.S467419

Reactivity：Mouse

Sample Type：4T1 cell-Xenograft
Soluble dietary fiber from Dendrocalamus brandisii (Munro) Kurz shoot improves liver injury by regulating gut microbial disorder in mice

IF: 6.5

Journal： Food Chemistry-X (2024)

DOI：10.1016/j.fochx.2024.101472

Reactivity：Mouse

Sample Type：liver tissue
Comparative analysis of phenolic compounds in different thinned unripe kiwifruits and their biological functions

IF: 6.5

Journal： Food Chemistry-X (2024)

DOI：10.1016/j.fochx.2024.101815

Reactivity：Mouse
SCF/C-kit drives spermatogenesis disorder induced by abscopal effects of cranial irradiation in mice

IF: 6.2

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)

DOI：10.1016/j.ecoenv.2024.116504

Reactivity：Mouse

Sample Type：serum, testis , brain tissues
Maternal exposure to DON during lactation induces testicular toxicity in pubertal and adult offspring mice

IF: 6.2

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)

DOI：10.1016/j.ecoenv.2024.116468

Reactivity：Mouse

Sample Type：Testis tissue
Early biomarkers in the presymptomatic phase of cognitive impairment: changes in the endocannabinoidome and serotonergic pathways in Alzheimer's-prone mice after mTBI

IF: 6.2

Journal： Acta Neuropathologica Communications (2024)

DOI：10.1186/s40478-024-01820-0

Reactivity：Mouse

Sample Type：plasma
GAMG ameliorates silica-induced pulmonary inflammation and fibrosis via the regulation of EMT and NLRP3/TGF-β1/Smad signaling pathway

IF: 6.2

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)

DOI：10.1016/j.ecoenv.2024.117124

Reactivity：Mouse

Sample Type：bronchoalveolar lavage fluid (BALF)
A Pectic Polysaccharide from Lycium ruthenicum Murray Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice

IF: 6.2

Journal： Current Research in Food Science (2024)

DOI：10.1016/j.crfs.2024.100955

Reactivity：Mouse

Sample Type：serum,colon tissue

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