High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-M0009
文献（1043）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 4.69-300 pg/mL
灵敏度： 2.76 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	4.69-300 pg/mL
灵敏度	2.76 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

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IF: 10.5

Journal： JOURNAL OF CONTROLLED RELEASE (2025)

DOI：10.1016/j.jconrel.2025.113597

Reactivity：Mouse
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Journal： JOURNAL OF CONTROLLED RELEASE (2025)

DOI：10.1016/j.jconrel.2025.113679

Reactivity：Mouse

Sample Type：serum
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IF: 10.2

Journal： Materials Today Bio (2025)

DOI：10.1016/j.mtbio.2025.101983

Reactivity：Mouse
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IF: 10.2

Journal： Materials Today Bio (2025)

DOI：10.1016/j.mtbio.2025.101943

Reactivity：Mouse

Sample Type：serum
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Journal： Materials Today Bio (2025)

DOI：10.1016/j.mtbio.2025.101945

Reactivity：Mouse

Sample Type：colon tissue
Grancalcin Hydrogel Microneedle Patches Alleviate Sepsis via Modulation of Calcium Signaling to Augment Immune Cell Phagocytosis

IF: 10

Journal： Advanced Healthcare Materials (2025)

DOI：10.1002/adhm.202501218

Reactivity：Mouse

Sample Type：serum
ROS-responsive hydrogel for bone regeneration: Controlled dimethyl fumarate release to reduce inflammation and enhance osteogenesis

IF: 9.4

Journal： Acta Biomaterialia (2025)

DOI：10.1016/j.actbio.2025.02.026

Reactivity：Mouse
Maternal high-fat diet exacerbates atherosclerosis development in offspring through epigenetic memory

IF: 9.4

Journal： Nature Cardiovascular Research (2025)

DOI：10.1038/s44161-025-00622-4

Reactivity：Mouse

Sample Type：plasma
Engineered tantalum sulfide nanosheets for effective acute liver injury treatment by regulating oxidative stress and inflammation

IF: 9.4

Journal： JOURNAL OF COLLOID AND INTERFACE SCIENCE (2025)

DOI：10.1016/j.jcis.2025.137596

Reactivity：Mouse

Sample Type：liver tissue
Inhibition of histone deacetylase 6 alleviates neuropathic pain via direct regulating post-translation of spinal STAT3 and decreasing downstream C-C Motif Chemokine Ligand 7 synthesis

IF: 9.3

Journal： Journal of Neuroinflammation (2025)

DOI：10.1186/s12974-025-03400-y

Reactivity：Mouse

Sample Type：L4-6 spinal cord tissue

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