High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：高敏型小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 4.69-300 pg/mL
- 灵敏度： 2.76 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 4.69-300 pg/mL |
| 灵敏度 | 2.76 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Bifidobacteria-Fermented Red Ginseng and Its Constituents Ginsenoside Rd and Protopanaxatriol Alleviate Anxiety/Depression in Mice by the Amelioration of Gut Dysbiosis
IF: 4.546 -
Chinese Propolis Prevents Obesity and Metabolism Syndromes Induced by a High Fat Diet and Accompanied by an Altered Gut Microbiota Structure in Mice
IF: 4.546 -
Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis
IF: 4.545 -
MG-132 attenuates cardiac deterioration of viral myocarditis via AMPK pathway
IF: 4.545 -
Dihydrotanshinone attenuates chemotherapy-induced intestinal mucositis and alters fecal microbiota in mice
IF: 4.545 -
Endothelial progenitor cell–derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes
IF: 4.545 -
Study on the protective effect and mechanism of Dicliptera chinensis (L.) Juss (Acanthaceae) polysaccharide on immune liver injury induced by LPS
IF: 4.545Journal： BIOMEDICINE & PHARMACOTHERAPY (2020)PMID：33370627Reactivity：MouseSample Type：Tissue homogenate -
Polysaccharides from Dicliptera chinensis ameliorate liver disturbance by regulating TLR-4/NF-κB and AMPK/Nrf2 signalling pathways
IF: 4.486Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020)PMID：32337831Reactivity：MouseSample Type：Serum,Tissue homogenate -
Defibrotide inhibits donor leucocyte-endothelial interactions and protects against acute graft-versus-host disease
IF: 4.486Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020)PMID：32519822Reactivity：MouseSample Type：Plasma -
MG53 Protects hUC-MSCs against Inflammatory Damage and Synergistically Enhances Their Efficacy in Neuroinflammation Injured Brain through Inhibiting NLRP3/Caspase-1/IL-1β Axis
IF: 4.486
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
