High Sensitivity Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：高敏型小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 4.69-300 pg/mL
- 灵敏度： 2.76 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 4.69-300 pg/mL |
| 灵敏度 | 2.76 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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Kupffer cell inactivation ameliorates immune liver injury via TNF-α/TNFR1 signal pathway in trichloroethylene sensitized mice
IF: 2.352Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2020)PMID：32811237Reactivity：MouseSample Type：Serum -
Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2
IF: 2.276Journal： Biomed Research International (2020)PMID：32934963Reactivity：MouseSample Type：Tissue homogenate -
Frequent exposure to varied home cage sizes alters pain sensitivity and some key inflammation-related biomarkers
IF: 2.214Journal： JOURNAL OF NEUROSCIENCE METHODS (2020)PMID：32768413Reactivity：MouseSample Type：Tissue homogenate -
Coenzyme Q10 alleviates sevoflurane?induced neuroinflammation by regulating the levels of apolipoprotein E and phosphorylated tau protein in mouse hippocampal neurons
IF: 2.100Journal： Molecular Medicine Reports (2020)PMID：32377738Reactivity：MouseSample Type：Tissue homogenate -
Placenta?derived mesenchymal stem cells ameliorate lipopolysaccharide?induced inflammation in RAW264.7 cells and acute lung injury in rats
IF: 2.100Journal： Molecular Medicine Reports (2020)PMID：32626979Reactivity：Mouse,RatSample Type：Cell culture supernatant -
Astaxanthin suppresses lipopolysaccharide?induced myocardial injury by regulating MAPK and PI3K/AKT/mTOR/GSK3β signaling
IF: 2.100 -
Osthole inhibits proliferation of kainic acid?activated BV?2 cells by modulating the Notch signaling pathway
IF: 2.100Journal： Molecular Medicine Reports (2020)PMID：33000274Reactivity：MouseSample Type：Cell culture supernatant -
MicroRNA-345-5p regulates depression by targeting suppressor of cytokine signaling 1
IF: 2.091Journal： Brain and Behavior (2020)PMID：32730696Reactivity：MouseSample Type：Cell culture supernatant -
The effectiveness of Hedyotis diffusa Willd extract in a mouse model of experimental autoimmune prostatitis
IF: 1.951 -
The Effects of RKI-1447 in a Mouse Model of Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet and in HepG2 Human Hepatocellular Carcinoma Cells Treated with Oleic Acid
IF: 1.918Journal： MEDICAL SCIENCE MONITOR (2020)PMID：32026851Reactivity：MouseSample Type：Serum,Cell culture supernatant,Tissue homogenate
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