High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-R0001
文献（1284）
说明书

说明书

别称：

Tnf、Tnfa、Tnfsf2

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Rat
检测范围： 1.56-100 pg/mL
灵敏度： 0.86 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法;ELISA;夹心法
检测范围	1.56-100 pg/mL
灵敏度	0.86 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Study on HIF-PHI combined with iron supplement in treatment of renal anemia in rats

IF: 2.2

Journal： BMC Nephrology (2025)

DOI：10.1186/s12882-025-04045-y

Reactivity：Rat

Sample Type：Serum
Sanggenol L inhibits the HMGB1/TLR4/NF-κB signaling pathway to prevent cerebral ischemia-reperfusion damage

IF: 2.2

Journal： JOURNAL OF MOLECULAR HISTOLOGY (2025)

DOI：10.1007/s10735-025-10472-w

Reactivity：Rat

Sample Type：Serum
Prophylactic role of omega 3 fatty acids in bisphenol F-induced sexual and erectile dysfunction is associated with penile redox homeostasis

IF: 2.2

Journal： Biochemistry and Biophysics Reports (2025)

DOI：10.1016/j.bbrep.2025.102103

Reactivity：Rat

Sample Type：Penile tissue
Cranberry extract attenuates indomethacin-induced gastriculcer in rats via its potential atioxidant and anti-inflammatory effects

IF: 2.2

Journal： JOURNAL OF MOLECULAR HISTOLOGY (2025)

DOI：10.1007/s10735-025-10438-y

Reactivity：Rat

Sample Type：serum
Antibiotic-Induced Gut Microbiome Dysbiosis Aggravates Cerebral Injury During Extracorporeal Membrane Oxygenation

IF: 2.1

Journal： CATHETERIZATION AND CARDIOVASCULAR INTERVENTIONS (2025)

DOI：10.1002/ccd.31589

Reactivity：Rat

Sample Type：Serum
Tacca chantrieri André Rhizome Extract Alleviates Scopolamine-Induced Cognitive Impairment and Neuroinflammation in Rats

IF: 2.1

Journal： Advances in Pharmacological and Pharmaceutical Sciences (2025)

DOI：10.1155/adpp/7334303

Reactivity：Rat

Sample Type：brain tissue
Therapeutic effects of Carissa edulis aqueous extract against L-glutamic acid-induced neurotoxicity in brain mice

IF: 2.0

Journal： IBRO Neuroscience Reports (2025)

DOI：10.1016/j.ibneur.2025.02.008

Reactivity：Mouse

Sample Type：brain tissue
The Effect of Inhaled Ozone Therapy in Two-Hit Rat Model of Lipopolysaccharides-Induced Acute Lung Injury and Bleomycin-Induced Pulmonary Fibrosis

IF: 1.9

Journal： PROTEIN JOURNAL (2025)

DOI：10.1007/s10930-024-10247-4

Reactivity：Rat

Sample Type：lung tissue
Effect of peripheral adiponectin on perioperative neurocognitive disorder via regulation of glucose metabolism in aged rats

IF: 1.6

Journal： NEUROREPORT (2025)

DOI：10.1097/WNR.0000000000002169

Reactivity：Rat

Sample Type：hippocampus tissue
LncRNA RMST contributes to neuroinflammation after traumatic brain injury by targeting miR-139-5p

IF: 1.5

Journal： FOLIA NEUROPATHOLOGICA (2025)

DOI：10.5114/fn.2024.144259

Reactivity：Rat

Sample Type：brain cortical tissue

Species

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Canine

Human

Mouse

Porcine

Rat

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