High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-R0001
文献（1284）
说明书

说明书

别称：

Tnf、Tnfa、Tnfsf2

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Rat
检测范围： 1.56-100 pg/mL
灵敏度： 0.86 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法;ELISA;夹心法
检测范围	1.56-100 pg/mL
灵敏度	0.86 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Elucidation of the Role of TRPV1, VEGF-A, TXA2, Redox Homeostasis, and Inflammatory Cascades in Protection against Cold Injuries by Herbosomal-Loaded PEG-Poloxamer Topical Formulation

IF: 4.6

Journal： ACS Applied Bio Materials (2024)

DOI：10.1021/acsabm.3c01197

Reactivity：Rat

Sample Type：skin tissue homogenate, blood plasma
Dexmedetomidine enhances Mitophagy via PINK1 to alleviate hippocampal neuronal Pyroptosis and improve postoperative cognitive dysfunction in elderly rat

IF: 4.6

Journal： EXPERIMENTAL NEUROLOGY (2024)

DOI：10.1016/j.expneurol.2024.114842

Reactivity：Rat

Sample Type：hippocampal tissues
Atractylodin modulates ASAH3L to improve galactose metabolism and inflammation to alleviate acute lung injury

IF: 4.6

Journal： iScience (2024)

DOI：10.1016/j.isci.2024.110751

Reactivity：Rat

Sample Type：bronchoalveolar lavage fluid (BALF),lung tissue
Methyltransferase METTL3 regulates neuropathic pain through m6A methylation modification of SOCS1

IF: 4.6

Journal： NEUROPHARMACOLOGY (2024)

DOI：10.1016/j.neuropharm.2024.110176

Reactivity：Rat

Sample Type：serum
3-Acetyl coumarin alleviate neuroinflammatory responses and oxidative stress in aluminum chloride-induced Alzheimer's disease rat model

IF: 4.6

Journal： INFLAMMOPHARMACOLOGY (2024)

DOI：10.1007/s10787-024-01434-x

Reactivity：Rat

Sample Type：brain tissue
Lactobacillus rhamnosus GG and Tannic Acid Synergistically Promote the Gut Barrier Integrity in a Rat Model of Experimental Diarrhea via Selective Immunomodulatory Cytokine Targeting

IF: 4.5

Journal： MOLECULAR NUTRITION & FOOD RESEARCH (2024)

DOI：10.1002/mnfr.202400295

Reactivity：Rat

Sample Type：ileal tissue
Pharmacological investigation of selected 1,2,4 triazole derivative against ethanol induced gastric ulcer

IF: 4.5

Journal： BIOORGANIC CHEMISTRY (2024)

DOI：10.1016/j.bioorg.2024.108040

Reactivity：Rat

Sample Type：gastric ulcer tissue
Diphenyl Diselenide Through Reduction of Inflammation, Oxidative Injury and Caspase-3 Activation Abates Doxorubicin-Induced Neurotoxicity in Rats

IF: 4.4

Journal： NEUROCHEMICAL RESEARCH (2024)

DOI：10.1007/s11064-023-04098-1
Studies Related to the Involvement of EsA in Improving Intestinal Inflammation in Acute Pancreatitis via the NF-κB Pathway

IF: 4.4

Journal： MEDIATORS OF INFLAMMATION (2024)

DOI：10.1155/2024/9078794

Reactivity：Rat

Sample Type：serum,intestinal mucosal
Cang-ai volatile oil alleviates nasal inflammation via Th1/Th2 cell imbalance regulation in a rat model of ovalbumin-induced allergic rhinitis

IF: 4.4

Journal： Frontiers in Pharmacology (2024)

DOI：10.3389/fphar.2024.1332036

Reactivity：Rat

Sample Type：Serum

Species

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