High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-R0001
文献（1284）
说明书

说明书

别称：

Tnf、Tnfa、Tnfsf2

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Rat
检测范围： 1.56-100 pg/mL
灵敏度： 0.86 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法;ELISA;夹心法
检测范围	1.56-100 pg/mL
灵敏度	0.86 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Sumac liposomes/mesenchymal stem cells fight methotrexate-induced nephrotoxicity in rats via regulating Nrf-2/Keap-1/HO-1 and apoptotic signaling pathways

IF: 4.3

Journal： ARCHIV DER PHARMAZIE (2024)

DOI：10.1002/ardp.202400684

Reactivity：Rat

Sample Type：kidney tissue
Evaluation of Hippocampal Microanatomy and Neuro-Biomarkers Following Administration of Silver Nanoparticles Conjugated with Tenofovir Disoproxil Fumarate in Experimental Diabetic Rats

IF: 4.3

Journal： Pharmaceuticals (2024)

DOI：10.3390/ph17121635

Reactivity：Rat

Sample Type：hippocampal tissue
Study on the effect and mechanism of Zhenzhu Tongluo pills in treating diabetic peripheral neuropathy injury

IF: 4.2

Journal： EUROPEAN JOURNAL OF MEDICAL RESEARCH (2024)

DOI：10.1186/s40001-024-01744-4
The antidepressant effects of protein arginine methyltransferase 2 involve neuroinflammation

IF: 4.2

Journal： NEUROCHEMISTRY INTERNATIONAL (2024)

DOI：10.1016/j.neuint.2024.105728
Research on the potential mechanism of Deapioplatycodin D against pulmonary fibrosis based on bioinformatics and experimental verification

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)

DOI：10.1016/j.ejphar.2024.176603

Reactivity：Rat

Sample Type：lung tissue
Transcriptomic Insights into Different Stimulation Intensity of Electroacupuncture in Treating COPD in Rat Models

IF: 4.2

Journal： Journal of Inflammation Research (2024)

DOI：10.2147/JIR.S458580

Reactivity：Rat

Sample Type：serum
Agmatine modulation of gut-brain axis alleviates dysbiosis-induced depression-like behavior in rats

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)

DOI：10.1016/j.ejphar.2024.176884

Reactivity：Rat

Sample Type：prefrontal cortex (PFC),hippocampus tissue
Effects of canagliflozin preconditioning on post-resuscitation myocardial function in a diabetic rat model of cardiac arrest and cardiopulmonary resuscitation

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)

DOI：10.1016/j.ejphar.2024.177212

Reactivity：Rat

Sample Type：serum
Modulation of Metabolic Pathways and Protection against Cadmium-Induced Disruptions with Taxifolin-Enriched Extract

IF: 4.1

Journal： ACS Omega (2024)

DOI：10.1021/acsomega.3c08989
Rutin Promotes Wound Healing by Inhibiting Oxidative Stress and Inflammation in Metformin-Controlled Diabetes in Rats

IF: 4.1

Journal： ACS Omega (2024)

DOI：10.1021/acsomega.3c05595

Species

{{relatedElisaPage.numTotal}} Results

All

Canine

Human

Mouse

Porcine

Rat

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual

Cat.No.：{{item.cat}}

{{index}}{{goods_show_value}}