High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-R0001
文献（1284）
说明书

说明书

别称：

Tnf、Tnfa、Tnfsf2

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Rat
检测范围： 1.56-100 pg/mL
灵敏度： 0.86 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法;ELISA;夹心法
检测范围	1.56-100 pg/mL
灵敏度	0.86 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Prevention of colon enlargement by TNF-α antagonist in a streptozotocin-induced diabetic rat model

IF: 2.5

Journal： HISTOLOGY AND HISTOPATHOLOGY (2024)

DOI：10.14670/HH-18-735

Reactivity：Rat

Sample Type：plasma
Effects of mid?gestational sevoflurane and magnesium sulfate on maternal oxidative stress, inflammation and fetal brain histopathology

IF: 2.4

Journal： Experimental and Therapeutic Medicine (2024)

DOI：10.3892/etm.2024.12574

Reactivity：Rat

Sample Type：serum
Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization

IF: 2.4

Journal： NEUROSCIENCE RESEARCH (2024)

DOI：10.1016/j.neures.2024.05.004

Reactivity：Rat

Sample Type：hippocampal tissue
Formulated Chitosan Microspheres Remodelled the Altered Gut Microbiota and Liver miRNA in Diet-Induced Type-2 Diabetic Rats

IF: 2.4

Journal： CARBOHYDRATE RESEARCH (2024)

DOI：10.1016/j.carres.2024.109301

Reactivity：Rat

Sample Type：serum
Ferulic acid nanoemulsion as a promising anti-ulcer tool: in vitro and in vivo assessment

IF: 2.4

Journal： DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY (2024)

DOI：10.1080/03639045.2024.2341786

Reactivity：Rat

Sample Type：serum
Physical exercise improved the hematological effect of vitamin D in type 2 diabetes mellitus-induced nephrotoxicity in rats

IF: 2.3

Journal： Biochemistry and Biophysics Reports (2024)

DOI：10.1016/j.bbrep.2024.101839

Reactivity：Rat

Sample Type：Renal tissue
Antioxidant and inflammatory-modulating properties of ginger and bitterleaf teas

IF: 2.2

Journal： INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2024)

DOI：10.1080/09603123.2024.2338894

Reactivity：Rat

Sample Type：plasma
Involvement of pentraxin-3 in the development of hypertension but not left ventricular hypertrophy in male spontaneously hypertensive rats

IF: 2.2

Journal： Physiological Reports (2024)

DOI：10.14814/phy2.70086

Reactivity：Rat

Sample Type：plasma
A Novel Machine Perfusion System for Enhancing Hepatic Microcirculation Perfusion

IF: 2.2

Journal： ARTIFICIAL ORGANS (2024)

DOI：10.1111/aor.14930

Reactivity：Rat

Sample Type：perfusate,plasma
Pancreastatin inhibitor PSTi8 improves ovarian health in Letrozole-HFD induced PCOS rats by ameliorating metabolic and reproductive parameters

IF: 2.1

Journal： STEROIDS (2024)

DOI：10.1016/j.steroids.2024.109558

Reactivity：Rat

Sample Type：serum

Species

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