High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：高敏型大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.86 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中TNF-α浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.86 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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Two flavonoids, baicalein and naringin, are effective as anti-inflammatory and anti-oxidant agents in a rat model of polymicrobial sepsis
IF: 3.300Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2023)PMID：36988563Reactivity：RatSample Type：serum -
Vinpocetine mitigates methotrexate-induced duodenal intoxication by modulating NF-κB, JAK1/STAT-3, and RIPK1/RIPK3/MLKL signals.
IF: 3.3Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2023)PMID：37493389Reactivity：RatSample Type：intestinal tissue -
Boric acid is associated with the suppression of apoptosis and endoplasmic reticulum stress in rat model of paracetamol -induced hepatotoxicity
IF: 3.3Journal： Journal of Taibah University for Science (2023)Reactivity：RatSample Type：liver,Serum -
Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation
IF: 3.200 -
Balancing renal Ang-II/Ang-(1–7) by xanthenone; an ACE2 activator; contributes to the attenuation of Ang-II/p38 MAPK/NF-κB p65 and Bax/caspase-3 pathways in amphotericin B-induced nephrotoxicity in rats
IF: 3.200 -
Remote preconditioning combined with nebulized budesonide alleviate lipopolysaccharide induced acute lung injury via regulating HO-1 and NF-κB in rats
IF: 3.200Journal： PULMONARY PHARMACOLOGY & THERAPEUTICS (2023)PMID：37060938Reactivity：RatSample Type：serum -
Neuroprotective responses of quercetin in regulation of biochemical, structural, and neurobehavioral effects in 28-day oral exposure of iron in rats
IF: 3.2 -
Estrogen ameliorates sepsis-induced vascular hyporeactivity in thoracic aorta of female rats via permissive effect of GRα expression
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：37002984Reactivity：RatSample Type：serum -
Adverse effects of an aldosterone synthase (CYP11B2) inhibitor, fadrozole (FAD286), on inflamed rat colon
IF: 3.1Journal： BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY (2023)PMID：37345281Reactivity：RatSample Type：distal colon,adrenal gland -
L-F001, a multifunctional fasudil–lipoic acid dimer, antagonizes hypoxic–ischemic brain damage by inhibiting the TLR4/MyD88 signaling pathway
IF: 3.1
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