High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：高敏型大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.86 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中TNF-α浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.86 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Selenium and zinc supplementation mitigates metals-(loids) mixture- mediated cardiopulmonary toxicity via attenuation of antioxidant, anti-inflammatory and antiapoptotic mechanisms in female Sprague Dawley rats
IF: 2.1Journal： Toxicology Research (2023)PMID：38179003PMC10762678Reactivity：RatSample Type：heart tissue,lung tissue -
TBC1 domain family member 25 protects against myocardial apoptosis and the proinflammatory response triggered by ischemia–reperfusion injury through suppression of the TAK1-JNK/p38 MAPK signaling cascade
IF: 2.1Journal： IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2023)PMID：38100060Reactivity：RatSample Type：plasma -
Alleviation of adipose-hepatic glycolipid dysregulation by acetate in experimental PCOS model is associated with NF-κB/NLRP3 repression
IF: 2.1Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)Reactivity：RatSample Type：adipose tissue,hepatic tissue -
Amaranthus spinosus (Spiny Pigweed) methanol leaf extract alleviates oxidative and inflammation induced by doxorubicin in male sprague dawley rats
IF: 2.000Journal： Advances in Traditional Medicine (2023)Reactivity：RatSample Type：serum -
Common salt aggravated pathology of testosterone-induced benign prostatic hyperplasia in adult male Wistar rat
IF: 2.0 -
9-POHSA prevents NF-kB activation and ameliorates LPS-induced inflammation in rat hepatocytes
IF: 1.9 -
Vanillic Acid Alleviates Right Ventricular Function in Rats With MCT-Induced Pulmonary Arterial Hypertension
IF: 1.800Journal： Natural Product Communications (2023)Reactivity：RatSample Type：serum -
The effects of Spirulina platensis on behavior in adolescent rats fed a high-fat diet
IF: 1.8Journal： INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE (2023)PMID：37315948Reactivity：RatSample Type：brain tissue -
Weissella confusa F213 ameliorated inflammation and maintained intestinal mucosa integrity in chemically induced colitis rats
IF: 1.8 -
Investigating the association between diet-induced “leaky gut” and the development of prediabetes
IF: 1.8Journal： EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2023)PMID：37751850Reactivity：RatSample Type：plasma
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
