High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：高敏型大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-HSEL-R0001
文献（1284）
说明书

说明书

别称：

Tnf、Tnfa、Tnfsf2

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3200.00 ¥2200.00 ¥14400.00

Size:

96 T 48 T 96 T × 5

反应性： Rat
检测范围： 1.56-100 pg/mL
灵敏度： 0.86 pg/mL

购买1个96 T 一步法/高敏型ELISA试剂盒减150元 购买1个48 T 一步法/高敏型ELISA试剂盒减50元

2025-08-19 ~ 2025-09-30

系列介绍	Elabscience^®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。
用途	试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法;ELISA;夹心法
检测范围	1.56-100 pg/mL
灵敏度	0.86 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Sinomenine alleviates experimental autoimmune uveitis in rats: Possible involvement of PI3K/AKT and NF-κB signaling pathways

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2025)

DOI：10.1016/j.ejphar.2025.177571

Reactivity：Rat

Sample Type：serum
JAK1/JAK2 ?NH?B?TOR BAR?C?T?N?B AMEL?ORATES SEPS?S-?NDUCED ACUTE K?DNEY ?NJURY ?N RATS

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2025)

DOI：10.1016/j.ejphar.2025.177770

Reactivity：Rat

Sample Type：Serum
Protective Effects of COG133 on Carbon Tetrachloride-Induced Acute Liver Injury: Modulation of Inflammation, Apoptosis and Sphingolipid Metabolism

IF: 4.2

Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2025)

DOI：10.1111/jcmm.70677

Reactivity：Rat

Sample Type：liver tissue
Xuebijing Injection Alleviates Sepsis-Induced Acute Lung Injury by Inhibition of Cell Apoptosis and Inflammation Through the Hippo Pathway

IF: 4.1

Journal： Journal of Inflammation Research (2025)

DOI：10.2147/JIR.S523991

Reactivity：Rat

Sample Type：lung tissue,serum
Regional molecular changes in chronic Lipopolysaccharide-induced neuroinflammation

IF: 4

Journal： Biological Psychiatry: Global Open Science (2025)

DOI：10.1016/j.bpsgos.2025.100515

Reactivity：Rat

Sample Type：serum
Vitamin D and omega-3 fatty acids attenuate MSG-induced neurodegeneration by modulating tau pathology, neuroinflammation, and VDR expression in rats

IF: 3.9

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-025-06109-3

Reactivity：Rat

Sample Type：hippocampal tissue
Astragaloside IV attenuates cadmium induced nephrotoxicity in rats by activating Nrf2

IF: 3.8

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-025-86312-4

Reactivity：Rat

Sample Type：kidney tissue,serum
Gallic and glycyrrhetinic acids prevent azithromycin-induced liver damage in rats by mitigating oxidative stress and inflammation

IF: 3.8

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-025-93120-3

Reactivity：Rat

Sample Type：liver tissue
The antifibrotic effect of Vildagliptin and Diaminodiphenyl Sulfone in murine schistosomiasis mansoni

IF: 3.8

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-025-91955-4

Reactivity：Mouse

Sample Type：liver tissue
Memantine abrogates testicular dysfunction induced by risperidone in rats with a potential role of ERK1/2-Nrf2-caspase-3 signaling pathway

IF: 3.8

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-025-94760-1

Reactivity：Rat

Sample Type：testicular tissue

Species

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Canine

Human

Mouse

Porcine

Rat

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